Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine

Kai Hong Guo; Kun Hua Lu; Yi Chun Yeh

doi:10.1021/acssynbio.8b00416

Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine

Kai Hong Guo
, Kun Hua Lu
, Yi Chun Yeh^*

^*此作品的通信作者

化學系

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

7 引文斯高帕斯（Scopus）

摘要

Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.

原文	英語
頁（從 - 到）	2790-2795
頁數	6
期刊	ACS Synthetic Biology
卷	7
發行號	12
DOIs	https://doi.org/10.1021/acssynbio.8b00416
出版狀態	已發佈 - 2018 11月 12

ASJC Scopus subject areas

生物醫學工程
生物化學、遺傳與分子生物學（雜項）

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10.1021/acssynbio.8b00416

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@article{f67c303764f1469c80fd801951021113,

title = "Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine",

abstract = "Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.",

keywords = "aromatic acids, auxin, E. coli, FeaR regulon, phenylacetic acid, phenylethylamine",

author = "Guo, \{Kai Hong\} and Lu, \{Kun Hua\} and Yeh, \{Yi Chun\}",

note = "Funding Information: This work was funded by the Ministry of Science and Technology of Taiwan under the Project No. 105-2113-M-003-013-MY2 and 107-2113-M-003-013-MY3. We thank Molecular Imaging Core Facility of National Taiwan Normal University for the assistance in high-content fluorescence microscopes. Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

year = "2018",

month = nov,

day = "12",

doi = "10.1021/acssynbio.8b00416",

language = "English",

volume = "7",

pages = "2790--2795",

journal = "ACS Synthetic Biology",

issn = "2161-5063",

publisher = "American Chemical Society",

number = "12",

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TY - JOUR

T1 - Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine

AU - Guo, Kai Hong

AU - Lu, Kun Hua

AU - Yeh, Yi Chun

N1 - Funding Information: This work was funded by the Ministry of Science and Technology of Taiwan under the Project No. 105-2113-M-003-013-MY2 and 107-2113-M-003-013-MY3. We thank Molecular Imaging Core Facility of National Taiwan Normal University for the assistance in high-content fluorescence microscopes. Publisher Copyright: © 2018 American Chemical Society.

PY - 2018/11/12

Y1 - 2018/11/12

N2 - Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.

AB - Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.

KW - aromatic acids

KW - auxin

KW - E. coli

KW - FeaR regulon

KW - phenylacetic acid

KW - phenylethylamine

UR - https://www.scopus.com/pages/publications/85057003542

UR - https://www.scopus.com/pages/publications/85057003542#tab=citedBy

U2 - 10.1021/acssynbio.8b00416

DO - 10.1021/acssynbio.8b00416

M3 - Article

C2 - 30418753

AN - SCOPUS:85057003542

SN - 2161-5063

VL - 7

SP - 2790

EP - 2795

JO - ACS Synthetic Biology

JF - ACS Synthetic Biology

IS - 12

ER -

Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine

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