Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine

Kai Hong Guo; Kun Hua Lu; Yi Chun Yeh

doi:10.1021/acssynbio.8b00416

Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine

Kai Hong Guo, Kun Hua Lu, Yi Chun Yeh

研究成果: 雜誌貢獻 › 文章 › 同行評審

1 引文斯高帕斯（Scopus）

摘要

Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.

原文	英語
頁（從 - 到）	2790-2795
頁數	6
期刊	ACS Synthetic Biology
卷	7
發行號	12
DOIs	https://doi.org/10.1021/acssynbio.8b00416
出版狀態	已發佈 - 2018 十一月 12

ASJC Scopus subject areas

Biomedical Engineering
Biochemistry, Genetics and Molecular Biology (miscellaneous)

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10.1021/acssynbio.8b00416

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title = "Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine",

abstract = "Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.",

keywords = "aromatic acids, auxin, E. coli, FeaR regulon, phenylacetic acid, phenylethylamine",

author = "Guo, {Kai Hong} and Lu, {Kun Hua} and Yeh, {Yi Chun}",

note = "Funding Information: This work was funded by the Ministry of Science and Technology of Taiwan under the Project No. 105-2113-M-003-013-MY2 and 107-2113-M-003-013-MY3. We thank Molecular Imaging Core Facility of National Taiwan Normal University for the assistance in high-content fluorescence microscopes.",

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AU - Guo, Kai Hong

AU - Lu, Kun Hua

AU - Yeh, Yi Chun

N1 - Funding Information: This work was funded by the Ministry of Science and Technology of Taiwan under the Project No. 105-2113-M-003-013-MY2 and 107-2113-M-003-013-MY3. We thank Molecular Imaging Core Facility of National Taiwan Normal University for the assistance in high-content fluorescence microscopes.

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AB - Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.

KW - aromatic acids

KW - auxin

KW - E. coli

KW - FeaR regulon

KW - phenylacetic acid

KW - phenylethylamine

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