Caspase-8 acts as a key upstream executor of mitochondria during justicidin A-induced apoptosis in human hepatoma cells

Chun Li Su; Lynn L.H. Huang; Li Min Huang; Jenq Chang Lee; Chun Nan Lin; Shen Jeu Won

doi:10.1016/j.febslet.2006.04.085

Caspase-8 acts as a key upstream executor of mitochondria during justicidin A-induced apoptosis in human hepatoma cells

Chun Li Su, Lynn L.H. Huang, Li Min Huang, Jenq Chang Lee, Chun Nan Lin, Shen Jeu Won^*

^*此作品的通信作者

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

40 引文斯高帕斯（Scopus）

摘要

Justicia procumbens is a traditional Taiwanese herbal remedy used to treat fever, pain, and cancer. Justicidin A, isolated from Justicia procumbens, has been reported to suppress in vitro growth of several tumor cell lines as well as hepatoma cells. In this study, justicidin A activated caspase-8 to increase tBid, disrupted mitochondrial membrane potential (Δψ_m), and caused the release of cytochrome c and Smac/DIABLO in Hep 3B and Hep G2 cells. Justicidin A also reduced Bcl-x_L and increased Bax and Bak in mitochondria. Caspase-8 inhibitor (Z-IETD) attenuated the justicidin A-induced disruption of Δψ_m. Growth of Hep 3B implanted in NOD-SCID mice was suppressed significantly by oral justicidin A (20 mg/kg/day). These results indicate that justicidin A-induced apoptosis in these cells proceeds via caspase-8 and is followed by mitochondrial disruption. Supplementary materials are available at http://myweb.ncku.edu.tw/~a725/.

原文	英語
頁（從 - 到）	3185-3191
頁數	7
期刊	FEBS Letters
卷	580
發行號	13
DOIs	https://doi.org/10.1016/j.febslet.2006.04.085
出版狀態	已發佈 - 2006 5月 29
對外發佈	是

ASJC Scopus subject areas

生物物理學
結構生物學
生物化學
分子生物學
遺傳學
細胞生物學

UN SDG

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10.1016/j.febslet.2006.04.085

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@article{f8a74ced72864d99af55aad8e1b92346,

title = "Caspase-8 acts as a key upstream executor of mitochondria during justicidin A-induced apoptosis in human hepatoma cells",

abstract = "Justicia procumbens is a traditional Taiwanese herbal remedy used to treat fever, pain, and cancer. Justicidin A, isolated from Justicia procumbens, has been reported to suppress in vitro growth of several tumor cell lines as well as hepatoma cells. In this study, justicidin A activated caspase-8 to increase tBid, disrupted mitochondrial membrane potential (Δψm), and caused the release of cytochrome c and Smac/DIABLO in Hep 3B and Hep G2 cells. Justicidin A also reduced Bcl-xL and increased Bax and Bak in mitochondria. Caspase-8 inhibitor (Z-IETD) attenuated the justicidin A-induced disruption of Δψm. Growth of Hep 3B implanted in NOD-SCID mice was suppressed significantly by oral justicidin A (20 mg/kg/day). These results indicate that justicidin A-induced apoptosis in these cells proceeds via caspase-8 and is followed by mitochondrial disruption. Supplementary materials are available at http://myweb.ncku.edu.tw/~a725/.",

keywords = "Apoptosis, Caspase, Hepatoma, Justicidin A, Mitochondria, Tumor growth",

author = "Su, {Chun Li} and Huang, {Lynn L.H.} and Huang, {Li Min} and Lee, {Jenq Chang} and Lin, {Chun Nan} and Won, {Shen Jeu}",

note = "Funding Information: This work was supported by a grant from National Science Council, Taipei, Taiwan, ROC (NSC 91-2320-B-006-075). ",

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doi = "10.1016/j.febslet.2006.04.085",

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journal = "FEBS Letters",

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T1 - Caspase-8 acts as a key upstream executor of mitochondria during justicidin A-induced apoptosis in human hepatoma cells

AU - Su, Chun Li

AU - Huang, Lynn L.H.

AU - Huang, Li Min

AU - Lee, Jenq Chang

AU - Lin, Chun Nan

AU - Won, Shen Jeu

N1 - Funding Information: This work was supported by a grant from National Science Council, Taipei, Taiwan, ROC (NSC 91-2320-B-006-075).

PY - 2006/5/29

Y1 - 2006/5/29

N2 - Justicia procumbens is a traditional Taiwanese herbal remedy used to treat fever, pain, and cancer. Justicidin A, isolated from Justicia procumbens, has been reported to suppress in vitro growth of several tumor cell lines as well as hepatoma cells. In this study, justicidin A activated caspase-8 to increase tBid, disrupted mitochondrial membrane potential (Δψm), and caused the release of cytochrome c and Smac/DIABLO in Hep 3B and Hep G2 cells. Justicidin A also reduced Bcl-xL and increased Bax and Bak in mitochondria. Caspase-8 inhibitor (Z-IETD) attenuated the justicidin A-induced disruption of Δψm. Growth of Hep 3B implanted in NOD-SCID mice was suppressed significantly by oral justicidin A (20 mg/kg/day). These results indicate that justicidin A-induced apoptosis in these cells proceeds via caspase-8 and is followed by mitochondrial disruption. Supplementary materials are available at http://myweb.ncku.edu.tw/~a725/.

AB - Justicia procumbens is a traditional Taiwanese herbal remedy used to treat fever, pain, and cancer. Justicidin A, isolated from Justicia procumbens, has been reported to suppress in vitro growth of several tumor cell lines as well as hepatoma cells. In this study, justicidin A activated caspase-8 to increase tBid, disrupted mitochondrial membrane potential (Δψm), and caused the release of cytochrome c and Smac/DIABLO in Hep 3B and Hep G2 cells. Justicidin A also reduced Bcl-xL and increased Bax and Bak in mitochondria. Caspase-8 inhibitor (Z-IETD) attenuated the justicidin A-induced disruption of Δψm. Growth of Hep 3B implanted in NOD-SCID mice was suppressed significantly by oral justicidin A (20 mg/kg/day). These results indicate that justicidin A-induced apoptosis in these cells proceeds via caspase-8 and is followed by mitochondrial disruption. Supplementary materials are available at http://myweb.ncku.edu.tw/~a725/.

KW - Apoptosis

KW - Caspase

KW - Hepatoma

KW - Justicidin A

KW - Mitochondria

KW - Tumor growth

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Caspase-8 acts as a key upstream executor of mitochondria during justicidin A-induced apoptosis in human hepatoma cells

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