C-terminal His-tagging results in substrate specificity changes of the thioesterase I from Escherichia coli

Ya Lin Lee, May Shyuan Su, Tai Huang Huang, Jei Fu Shaw

研究成果: 雜誌貢獻期刊論文同行評審

24 引文 斯高帕斯(Scopus)

摘要

The biochemical properties of Escherichia coli thioesterase I, His-tagged (HT) on the C-terminal, were systematically analyzed and compared with that without the His-tag (WT). These two types of enzymes exhibit similar optimal temperature and pH dependence, but subtle differences were detected. Kinetic studies revealed that the kcat/Km values of the HT enzyme for the substrates palmitoyl-CoA and p-nitrophenyl dodecanoate were 36- and 10-fold lower than those of the WT, respectively. In contrast, HT had a fivefold increased catalytic efficiency for p-nitrophenyl acetate, and up to fourfold increases toward phenylalanine- and tyrosine-derived ester substrates, L-NBPNPE (N-carbobenzoxy-L-phenylalanine p-nitrophenyl ester) and L-NBTNPE (N-carbobenzoxy-L-tyrosine p-nitrophenyl ester), respectively. For L-NBPNPE and L-NBTNPE, the increases were attributed to the higher kcat values with little changes in Km, whereas the increase for p-nitrophenyl acetate was mainly attributed to the lower Km value. It is concluded that addition of six hydrophilic histidine residues on the C-terminus resulted in a change in substrate specificity of E. coli thioesterase I toward more hydrophilic substrates.

原文英語
頁(從 - 到)1113-1118
頁數6
期刊JAOCS, Journal of the American Oil Chemists' Society
76
發行號10
DOIs
出版狀態已發佈 - 1999
對外發佈

ASJC Scopus subject areas

  • 一般化學工程
  • 有機化學

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