Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein

H. E. Horng; S. Y. Yang; Chin Yih Hong; C. M. Liu; P. S. Tsai; H. C. Yang; C. C. Wu

doi:10.1063/1.2207990

Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein

H. E. Horng^*
, S. Y. Yang
, Chin Yih Hong
, C. M. Liu
, P. S. Tsai
, H. C. Yang
, C. C. Wu

^*此作品的通信作者

光電工程研究所暨學士學位學程

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

56 引文斯高帕斯（Scopus）

摘要

In this work, we developed immunomagnetic detection techniques for detecting human C-reactive protein (CRP). To enhance the technique's sensitivity to human CRP, biofunctionalized magnetic nanoparticles were used as markers, and a superconductive quantum interference device gradiometer system was adapted to measure the saturated magnetization of magnetically labeled CRP-anti-CRP immune complexes. Sensitivity for human CRP was found to be as high as 1 ng in 0.1 ml, i.e., 10 ng/ml in concentration. This sensitivity is much higher than that of conventional enzyme linked immunosorbent assay by one order of magnitude.

原文	英語
文章編號	252506
期刊	Applied Physics Letters
卷	88
發行號	25
DOIs	https://doi.org/10.1063/1.2207990
出版狀態	已發佈 - 2006 6月 19

ASJC Scopus subject areas

物理與天文學（雜項）

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10.1063/1.2207990

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title = "Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein",

abstract = "In this work, we developed immunomagnetic detection techniques for detecting human C-reactive protein (CRP). To enhance the technique's sensitivity to human CRP, biofunctionalized magnetic nanoparticles were used as markers, and a superconductive quantum interference device gradiometer system was adapted to measure the saturated magnetization of magnetically labeled CRP-anti-CRP immune complexes. Sensitivity for human CRP was found to be as high as 1 ng in 0.1 ml, i.e., 10 ng/ml in concentration. This sensitivity is much higher than that of conventional enzyme linked immunosorbent assay by one order of magnitude.",

author = "Horng, \{H. E.\} and Yang, \{S. Y.\} and Hong, \{Chin Yih\} and Liu, \{C. M.\} and Tsai, \{P. S.\} and Yang, \{H. C.\} and Wu, \{C. C.\}",

note = "Funding Information: This work is supported by the National Science Council of Taiwan under Grant Nos. 94-2120-M-003-002, 94-2112-M-003-018, 94-2112-M-003-004, and 94-2212-E-001, 95-2752-M-002-016-PAE.",

year = "2006",

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doi = "10.1063/1.2207990",

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AU - Horng, H. E.

AU - Yang, S. Y.

AU - Hong, Chin Yih

AU - Liu, C. M.

AU - Tsai, P. S.

AU - Yang, H. C.

AU - Wu, C. C.

N1 - Funding Information: This work is supported by the National Science Council of Taiwan under Grant Nos. 94-2120-M-003-002, 94-2112-M-003-018, 94-2112-M-003-004, and 94-2212-E-001, 95-2752-M-002-016-PAE.

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N2 - In this work, we developed immunomagnetic detection techniques for detecting human C-reactive protein (CRP). To enhance the technique's sensitivity to human CRP, biofunctionalized magnetic nanoparticles were used as markers, and a superconductive quantum interference device gradiometer system was adapted to measure the saturated magnetization of magnetically labeled CRP-anti-CRP immune complexes. Sensitivity for human CRP was found to be as high as 1 ng in 0.1 ml, i.e., 10 ng/ml in concentration. This sensitivity is much higher than that of conventional enzyme linked immunosorbent assay by one order of magnitude.

AB - In this work, we developed immunomagnetic detection techniques for detecting human C-reactive protein (CRP). To enhance the technique's sensitivity to human CRP, biofunctionalized magnetic nanoparticles were used as markers, and a superconductive quantum interference device gradiometer system was adapted to measure the saturated magnetization of magnetically labeled CRP-anti-CRP immune complexes. Sensitivity for human CRP was found to be as high as 1 ng in 0.1 ml, i.e., 10 ng/ml in concentration. This sensitivity is much higher than that of conventional enzyme linked immunosorbent assay by one order of magnitude.

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Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein

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