Bioassay using blocking temperature: Interparticle interactions between biofunctionalized magnetic nanoparticles conjugated with biotargets

C. Y. Wang; T. W. Yang; D. Shen; K. L. Chen; J. M. Chen; S. H. Liao; J. J. Chieh; H. C. Yang; L. M. Wang

doi:10.1063/1.4979142

Bioassay using blocking temperature: Interparticle interactions between biofunctionalized magnetic nanoparticles conjugated with biotargets

C. Y. Wang, T. W. Yang, D. Shen, K. L. Chen^*, J. M. Chen, S. H. Liao, J. J. Chieh, H. C. Yang, L. M. Wang

^*此作品的通信作者

光電工程學士學位學程

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

4 引文斯高帕斯（Scopus）

摘要

This paper reports a bioassay of alpha-fetoprotein (AFP) concentration achieved via the measurement of blocking temperature (T_B). Biofunctionalized magnetic nanoparticles (BMNs) consisting of anti-alpha-fetoprotein coated onto dextran-coated magnetic nanoparticles composed of Fe₃O₄ were prepared and then conjugated with AFP biotargets. It was found that both the saturation magnetization and value of T_B increased with the concentration of the associated AFP. Furthermore, the dependence of T_B of the samples on magnetic field agreed with the interparticle interaction model. Thus, this study demonstrated a platform to detect biomarkers by characterizing T_B with a sensitivity limit of 20 ppb of AFP. The promising results obtained for this bioassay can be attributed to the interparticle interactions and Néel motions of magnetic moments in the BMNs.

原文	英語
文章編號	133701
期刊	Applied Physics Letters
卷	110
發行號	13
DOIs	https://doi.org/10.1063/1.4979142
出版狀態	已發佈 - 2017 3月 27

ASJC Scopus subject areas

物理與天文學（雜項）

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10.1063/1.4979142

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title = "Bioassay using blocking temperature: Interparticle interactions between biofunctionalized magnetic nanoparticles conjugated with biotargets",

abstract = "This paper reports a bioassay of alpha-fetoprotein (AFP) concentration achieved via the measurement of blocking temperature (TB). Biofunctionalized magnetic nanoparticles (BMNs) consisting of anti-alpha-fetoprotein coated onto dextran-coated magnetic nanoparticles composed of Fe3O4 were prepared and then conjugated with AFP biotargets. It was found that both the saturation magnetization and value of TB increased with the concentration of the associated AFP. Furthermore, the dependence of TB of the samples on magnetic field agreed with the interparticle interaction model. Thus, this study demonstrated a platform to detect biomarkers by characterizing TB with a sensitivity limit of 20 ppb of AFP. The promising results obtained for this bioassay can be attributed to the interparticle interactions and N{\'e}el motions of magnetic moments in the BMNs.",

author = "Wang, {C. Y.} and Yang, {T. W.} and D. Shen and Chen, {K. L.} and Chen, {J. M.} and Liao, {S. H.} and Chieh, {J. J.} and Yang, {H. C.} and Wang, {L. M.}",

note = "Publisher Copyright: {\textcopyright} 2017 Author(s).",

year = "2017",

month = mar,

day = "27",

doi = "10.1063/1.4979142",

language = "English",

volume = "110",

journal = "Applied Physics Letters",

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TY - JOUR

T1 - Bioassay using blocking temperature

T2 - Interparticle interactions between biofunctionalized magnetic nanoparticles conjugated with biotargets

AU - Wang, C. Y.

AU - Yang, T. W.

AU - Shen, D.

AU - Chen, K. L.

AU - Chen, J. M.

AU - Liao, S. H.

AU - Chieh, J. J.

AU - Yang, H. C.

AU - Wang, L. M.

PY - 2017/3/27

Y1 - 2017/3/27

N2 - This paper reports a bioassay of alpha-fetoprotein (AFP) concentration achieved via the measurement of blocking temperature (TB). Biofunctionalized magnetic nanoparticles (BMNs) consisting of anti-alpha-fetoprotein coated onto dextran-coated magnetic nanoparticles composed of Fe3O4 were prepared and then conjugated with AFP biotargets. It was found that both the saturation magnetization and value of TB increased with the concentration of the associated AFP. Furthermore, the dependence of TB of the samples on magnetic field agreed with the interparticle interaction model. Thus, this study demonstrated a platform to detect biomarkers by characterizing TB with a sensitivity limit of 20 ppb of AFP. The promising results obtained for this bioassay can be attributed to the interparticle interactions and Néel motions of magnetic moments in the BMNs.

AB - This paper reports a bioassay of alpha-fetoprotein (AFP) concentration achieved via the measurement of blocking temperature (TB). Biofunctionalized magnetic nanoparticles (BMNs) consisting of anti-alpha-fetoprotein coated onto dextran-coated magnetic nanoparticles composed of Fe3O4 were prepared and then conjugated with AFP biotargets. It was found that both the saturation magnetization and value of TB increased with the concentration of the associated AFP. Furthermore, the dependence of TB of the samples on magnetic field agreed with the interparticle interaction model. Thus, this study demonstrated a platform to detect biomarkers by characterizing TB with a sensitivity limit of 20 ppb of AFP. The promising results obtained for this bioassay can be attributed to the interparticle interactions and Néel motions of magnetic moments in the BMNs.

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DO - 10.1063/1.4979142

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SN - 0003-6951

VL - 110

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 13

M1 - 133701

ER -

Bioassay using blocking temperature: Interparticle interactions between biofunctionalized magnetic nanoparticles conjugated with biotargets

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