Automatic calibration of the spatial position and orientation for the tomographic digital holographic microscopy

Li Chien Lin, Chau Jern Cheng, Yu Chun Hu

研究成果: 書貢獻/報告類型會議論文篇章

摘要

In previous study, a tomographic imaging system is built up for measuring the three-dimensional refractive index distribution inside the micrometer-sized biological cell by optically driven full-angle rotation scheme based on digital holographic microscopy, named as optical-driven tomographic DHM (OT-DHM) system. However, a small perturbation of the system will lead the inaccurate of the positions and the orientation of the micrometer-sized sample, thus the automatic calibration of the reconstructed phase images in the OT-DHM system is required. For this purpose, a novel model-based algorithm is proposed, in which we employ a 3-D ellipse shape for modeling the samples. The parameters of the ellipse-like shape on a small number of the projections are estimated and used them to build up the 3-D ellipse model of the samples. In advance, the reconstructed phase images are highly contaminated by the uneven background and coherence speckle noise. The block-based between-class criterion is used to suppress the effect of the non-uniform background, and the anisotropic diffusion process is utilized for the noise cleaning, including shot noise and speckles noise on the reconstructed phase. The boundary of the cell in each projection can be considered as the 2-D ellipse, and used to estimate the parameters of the 2-D ellipse. The established 3-D ellipse shape is applied for the calibration of the spatial positions and the orientations of the all other rotational angles. With the automatic calibration algorithm, the OT-DHM system can effectively reconstructed the three-dimensional refractive index distribution inside the micrometer-sized samples.

原文英語
主出版物標題Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV
編輯Pietro Ferraro, Simonetta Grilli, Monika Ritsch-Marte, Monika Ritsch-Marte, Christoph K. Hitzenberger
發行者SPIE
ISBN(電子)9781510627994
DOIs
出版狀態已發佈 - 2019 一月 1
事件Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV 2019 - Munich, 德国
持續時間: 2019 六月 242019 六月 26

出版系列

名字Proceedings of SPIE - The International Society for Optical Engineering
11060
ISSN(列印)0277-786X
ISSN(電子)1996-756X

會議

會議Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV 2019
國家/地區德国
城市Munich
期間2019/06/242019/06/26

ASJC Scopus subject areas

  • 電子、光磁材料
  • 凝聚態物理學
  • 電腦科學應用
  • 應用數學
  • 電氣與電子工程

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