Application of digital holographic tomography in antitumor effect of cantharides complex on 4T1 breast cancer cells

Chen Wen Lu, Andrey V. Belashov, Anna A. Zhikhoreva, Irina V. Semenova, Chau Jern Cheng, Li Yu Su, Chung Hsin Wu*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

摘要

The study focuses on a methodology providing noninvasive monitoring and evaluation of the antitumor effect of traditional Chinese medicine, cantharides complex (canth), on 4T1 breast tumor cells.Digital holographic tomography (DHT) and developed data post-processing algorithms were used for quantitative estimation of changes in optical and morphological parameters of cells.We calculated and compared data on the refractive index, thickness, and projected area of 4T1 breast tumor cells in control untreated specimens and those treated with doxorubicin hydrochloride (DOX), canth, and their combinations. Post-treatment changes in cellular morphology recorded by DHT demonstrated that the two drugs led to noticeably different morphological changes in cells that can be presumably associated with different pathways of their death, apoptosis, or necrosis. The effect of combined treatment with these two drugs strongly depended on their relative concentrations and could lead to changes characteristic either for DOX or for canth; however, being more profound than those obtained when using each drug solely. The results obtained by DHT are in a good correspondence with commonly used cell viability analysis and immunofluorescent analysis of changes in cellular cytoskeleton.

原文英語
頁(從 - 到)3365-3373
頁數9
期刊Applied Optics
60
發行號12
DOIs
出版狀態已發佈 - 2021 四月 20

ASJC Scopus subject areas

  • 原子與分子物理與光學
  • 工程(雜項)
  • 電氣與電子工程

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