TY - JOUR
T1 - Application of capillary electrophoresis-fluorescence line-narrowing spectroscopy for on-line spectral characterization of closely related analytes
AU - Zamzow, D.
AU - Lin, C. H.
AU - Small, G. J.
AU - Jankowiak, R.
N1 - Funding Information:
Ames Laborato~1 is operated for the US Department of Energy by Iowa State University under contract no. W-7405-Eng-82. This work was supported by the Office of Health and Environmental Research, Office of Energy Research. One of us (C.-H. L.) was supported by the National Institute of Health, grant no. POI CA49210-05. The authors thank Dr. E.L. Cavalieri and Dr. K.-M. Li (Eppley Institute for Research in Cancer, Omaha, NE, USA) for providing us with the DNA-adduct samples.
PY - 1997/9/26
Y1 - 1997/9/26
N2 - Capillary electrophoresis (CE) interfaced with low-temperature (4.2 K) fluorescence line-narrowing spectroscopy (FLNS) is used for the separation and spectral characterization of closely related analytes. In this paper, the CE-FLNS system is applied to the analysis of a mixture of deuterated and protonated benzo[a]pyrene, a mixture of structurally similar benzo[a]pyrene and benzo[e]pyrene, and mixtures of dibenzo[a,l]pyrene-derived adenine DNA adducts. The CE-FLNS system provides on-line separation and high-resolution spectroscopic identification of CE-separated analytes, via fingerprint structure of vibrationally resolved FLN spectra at 4.2 K. The combination of the separation power of CE and the spectral selectivity of FLNS provide a methodology that has potential to become a powerful tool for molecular analyte characterization. The main applications of the CE-FLNS system, due to its selectivity, should be in the chemical analysis of structurally similar analytes and applications where analyte purity and detailed structural characterization are required.
AB - Capillary electrophoresis (CE) interfaced with low-temperature (4.2 K) fluorescence line-narrowing spectroscopy (FLNS) is used for the separation and spectral characterization of closely related analytes. In this paper, the CE-FLNS system is applied to the analysis of a mixture of deuterated and protonated benzo[a]pyrene, a mixture of structurally similar benzo[a]pyrene and benzo[e]pyrene, and mixtures of dibenzo[a,l]pyrene-derived adenine DNA adducts. The CE-FLNS system provides on-line separation and high-resolution spectroscopic identification of CE-separated analytes, via fingerprint structure of vibrationally resolved FLN spectra at 4.2 K. The combination of the separation power of CE and the spectral selectivity of FLNS provide a methodology that has potential to become a powerful tool for molecular analyte characterization. The main applications of the CE-FLNS system, due to its selectivity, should be in the chemical analysis of structurally similar analytes and applications where analyte purity and detailed structural characterization are required.
KW - Detection, electrophoresis
KW - DNA
KW - Fluorescence line-narrowing spectroscopy
KW - Polynuclear aromatic hydrocarbons
UR - http://www.scopus.com/inward/record.url?scp=0030720242&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030720242&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(97)00499-8
DO - 10.1016/S0021-9673(97)00499-8
M3 - Article
C2 - 9368378
AN - SCOPUS:0030720242
SN - 0021-9673
VL - 781
SP - 73
EP - 80
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -