Anti-inflammatory effect of charantadiol a, isolated from wild bitter melon leaf, on heat-inactivated porphyromonas gingivalis-stimulated thp-1 monocytes and a periodontitis mouse model

Tzung Hsun Tsai, Chi I. Chang, Ya Ling Hung, Wen Cheng Huang, Hsiang Chang, Yueh Hsiung Kuo, Jong Ho Chyuan, Lu Te Chuang*, Po Jung Tsai

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

摘要

Porphyromonas gingivalis has been identified as one of the major periodontal pathogens. Activity-directed fractionation and purification processes were employed to identify bioactive compounds from bitter melon leaf. Ethanolic extract of bitter melon leaf was separated into five subfrac-tions by open column chromatography. Subfraction-5-3 significantly inhibited P. gingivalis-induced interleukin (IL)-8 and IL-6 productions in human monocytic THP-1 cells and then was subjected to separation and purification by using different chromatographic methods. Consequently, 5β,19-epoxycucurbita-6,23(E),25(26)-triene-3β,19(R)-diol (charantadiol A) was identified and isolated from the subfraction-5-3. Charantadiol A effectively reduced P. gingivalis-induced IL-6 and IL-8 productions and triggered receptors expressed on myeloid cells (TREM)-1 mRNA level of THP-1 cells. In a separate study, charantadiol A significantly suppressed P. gingivalis-stimulated IL-6 and tumor necrosis factor-α mRNA levels in gingival tissues of mice, confirming the inhibitory effect against P. gingivalis-induced periodontal inflammation. Thus, charantadiol A is a potential anti-inflammatory agent for modulating P. gingivalis-induced inflammation.

原文英語
文章編號5651
期刊Molecules
26
發行號18
DOIs
出版狀態已發佈 - 2021 九月

ASJC Scopus subject areas

  • 分析化學
  • 化學(雜項)
  • 分子醫學
  • 藥學科學
  • 藥物發現
  • 物理與理論化學
  • 有機化學

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