@article{7270a1e538dc40fa8b95a2166485cf83,
title = "Analysis of Genetic Diversity of Two Intersterility Groups of Ganoderma australe by DNA Sequencing",
abstract = "本研究在以聚合酶鏈反應（PCR）和DNA定序法（DNA sequencing），探討南方靈芝Ganoderma australe（Fr.）Pat.種內的兩群雜交不孕性（intersterility group）的遺傳變異，並以不同亞屬的新日本靈芝G. neo-japonicum lmaz作為比較。以互補於酵母菌的rRNA基因的引子與DNA聚合酶，將rRNA基因重複單位的內部轉錄間隔片段（ITS 1、ITS 2）選殖出來。置入pGEM-T載體後，將篩選出的純系進行DNA序列分析。再以PAUP軟體，分析各樣品ITS 1、ITS 2序列的歧異度，建築樹狀演化圖。我們的結果顯示南方靈芝種內的兩群雜交不孕性已呈現顯著的遺傳分化。",
keywords = "南方靈芝, 雜交不孕性群, 內部轉錄間隔片段, DNA定序法, Ganoderma australe, intersterility group, internal transcribed spacer, DNA sequencing",
author = "君玉 張 and 增勇 葉 and 李, {桂楨(Guey-Jen Lee-Chen)}",
year = "1996",
doi = "10.29812/BBNTNU.199606.0007",
language = "繁體中文",
volume = "31",
pages = "47--53",
journal = "師大生物學報",
issn = "0378-8091",
publisher = "臺灣師範大學生命科學系",
number = "1",
}