An approach for differentiating uniform glutamatergic neurons from mouse embryonic stem cells

Jen Hua Chuang, Li Chu Tung, Guey Jen Lee-Chen, Yi Yin, Yenshou Lin*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

3 引文 斯高帕斯(Scopus)

摘要

Much effort is being marshaled to generate uniform neuronal populations from embryonic stem (ES) cells, but a completely reliable method has yet to be developed. Here we modified and established a method that brings us closer to this goal. By examining many parameters, we found that the optimal timing of applying a freshly made trypsin/EDTA (ethylenediaminetetraacetic acid) solution to dissociate embryoid bodies determines the success of the outcome. Analyses demonstrated that with this approach, more than 87% of cells differentiated into glutamatergic neurons. Hence, these uniform neurons that were differentiated from ES cells provide an ideal cellular model for many aspects of research.

原文英語
頁(從 - 到)149-151
頁數3
期刊Analytical Biochemistry
410
發行號1
DOIs
出版狀態已發佈 - 2011 三月 1

ASJC Scopus subject areas

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學

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