Ammonia excretion by the skin of zebrafish (Danio rerio) larvae

Tin Han Shih, Jiun Lin Horng, Pung Pung Hwang, Li-Yih Lin*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

116 引文 斯高帕斯(Scopus)

摘要

The mechanism of ammonia excretion in freshwater teleosts is not well understood. In this study, scanning ion-selective electrode technique was applied to measure H+ and NH4+ fluxes in specific cells on the skin of zebrafish larvae. NH4+ extrusion was relatively high in H+ pump-rich cells, which were identified as the H+-secreting ionocyte in zebrafish. Minor NH 4+ extrusion was also detected in keratinocytes and other types of ionocytes in larval skin. NH4+ extrusion from the skin was tightly linked to acid secretion. Increases in the external pH and buffer concentration (5 mM MOPS) diminished H+ and NH 4+ gradients at the larval surface. Moreover, coupled decreases in NH4+ and H+ extrusion were found in larvae treated with an H+-pump inhibitor (bafilomycin A1) or H+-pump gene (atp6v1a) knockdown. Knockdown of Rhcg1 with morpholino-oligonucleotides also decreased NH4+ excretion. This study demonstrates ammonia excretion in epithelial cells of larval skin through an acid-trapping mechanism, and it provides direct evidence for the involvement of the H+ pump and an Rh glycoprotein (Rhcg1) in ammonia excretion.

原文英語
期刊American Journal of Physiology - Cell Physiology
295
發行號6
DOIs
出版狀態已發佈 - 2008 十二月 1

ASJC Scopus subject areas

  • 細胞生物學
  • 生理學

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