TY - JOUR
T1 - Affinity capture surface carboxyl-functionalized MoS2 sheets to enhance the sensitivity of surface plasmon resonance immunosensors
AU - Chiu, Nan Fu
AU - Lin, Ting Li
N1 - Funding Information:
The authors would like to thank the Ministry of Science and Technology of the Republic of China, Taiwan, for financially supporting this research under Contract No. MOST 103–2221-E-003–008, MOST 104–2314-B-195-015, MOST 105–2221-E-003–027 and MOST 106–2221-E-003–020. We thank Dr. Yao-Jane Hsu groups for they help in analyzing XPS spectra (National Synchrotron Radiation Research Center, Beamline 09A2 and 24A1).
Funding Information:
The authors would like to thank the Ministry of Science and Technology of the Republic of China, Taiwan , for financially supporting this research under Contract No. MOST 103–2221-E-003–008 , MOST 104–2314-B-195-015 , MOST 105–2221-E-003–027 and MOST 106–2221-E-003–020 . We thank Dr. Yao-Jane Hsu groups for they help in analyzing XPS spectra (National Synchrotron Radiation Research Center, Beamline 09A2 and 24A1).
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - The development of functionalized molybdenum disulfide (MoS2) has led to a new trend in the biosensing field, owing to its high sensitivity and bio-affinity characteristics with regards to the simple synthesis of carboxyl-functionalized MoS2 nanocomposites. In this study, we used monochloroacetic acid (MCA) to successfully modify carboxyl-MoS2. The efficiency of this MCA modification method showed a higher -COOH group content of 30.1%, mainly due to chlorine atoms occupying the MoS2 sulfur vacancy to allow for the formation of a strong bonding effect. This then enhanced the surface area of -COOH and improved the formation of covalent bonds between proteins. We demonstrated that MoS2-COOH-based surface plasmon resonance (SPR) chips can provide excellent sensitivity and high affinity for immunoassay biomolecules detected in a low sample volume of 20 μl. With respect to the shifts of the SPR angles of the chips, the high binding affinity at a BSA concentration of 14.5 nM for a MoS2-COOH chip, a MoS2 chip and a traditional SPR chip are 4.69 m° 2.49 m° and 1.53 m° respectively. In addition, the MoS2-COOH chip could amplify the SPR angle response by 3.1 folds and enhance the high association rate of ka by 212 folds compared to MoS2 and traditional SPR chips. The results thus obtained revealed that the overall affinity binding value, KA, of the MoS2-COOH chip can be significantly enhanced by up to ∼ 6.5 folds that of the MoS2 chip. In summary, the excellent binding affinity, biocompatible and high sensitivity suggest the potential of the clinical application of this MoS2-COOH-based SPR chip detection method for in vitro diagnostic and point-of-care testing devices.
AB - The development of functionalized molybdenum disulfide (MoS2) has led to a new trend in the biosensing field, owing to its high sensitivity and bio-affinity characteristics with regards to the simple synthesis of carboxyl-functionalized MoS2 nanocomposites. In this study, we used monochloroacetic acid (MCA) to successfully modify carboxyl-MoS2. The efficiency of this MCA modification method showed a higher -COOH group content of 30.1%, mainly due to chlorine atoms occupying the MoS2 sulfur vacancy to allow for the formation of a strong bonding effect. This then enhanced the surface area of -COOH and improved the formation of covalent bonds between proteins. We demonstrated that MoS2-COOH-based surface plasmon resonance (SPR) chips can provide excellent sensitivity and high affinity for immunoassay biomolecules detected in a low sample volume of 20 μl. With respect to the shifts of the SPR angles of the chips, the high binding affinity at a BSA concentration of 14.5 nM for a MoS2-COOH chip, a MoS2 chip and a traditional SPR chip are 4.69 m° 2.49 m° and 1.53 m° respectively. In addition, the MoS2-COOH chip could amplify the SPR angle response by 3.1 folds and enhance the high association rate of ka by 212 folds compared to MoS2 and traditional SPR chips. The results thus obtained revealed that the overall affinity binding value, KA, of the MoS2-COOH chip can be significantly enhanced by up to ∼ 6.5 folds that of the MoS2 chip. In summary, the excellent binding affinity, biocompatible and high sensitivity suggest the potential of the clinical application of this MoS2-COOH-based SPR chip detection method for in vitro diagnostic and point-of-care testing devices.
KW - Biosensor
KW - Bovine serum albumin (BSA)
KW - Carboxyl-functionalized
KW - Molybdenum disulfide (MoS)
KW - Surface plasmon resonance (SPR)
UR - http://www.scopus.com/inward/record.url?scp=85044627048&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85044627048&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2018.03.073
DO - 10.1016/j.talanta.2018.03.073
M3 - Article
C2 - 29759186
AN - SCOPUS:85044627048
SN - 0039-9140
VL - 185
SP - 174
EP - 181
JO - Talanta
JF - Talanta
ER -