A RFID tag based remote DNA sensing system

J. H. Chien, C. Y. Tsai, Y. T. Cheng, C. R. Yang, P. Y. Wang, T. L. Chang, D. S. Lee, C. W. Chang, W. P. Chou, C. C. Chiang, Y. W. Lee, P. H. Chen

研究成果: 書貢獻/報告類型會議貢獻

摘要

Different from traditional detection methods including polymerase chain reaction (PCR) based assays and enzyme-linked immunosorbent assays (ELISA) which are expensive and time consuming for biochemical analysis, in our study, a DNA chip assay using gold nanoparticle (AuNP ) immunological amplification method and RFID communication platform were applied to detect DNA sequences rapidly and conveniently. First the capture DNA (cDNA) was immobilized to the substrate on which the capacitor locates. Then the target DNA (tDNA) and probe DNA (pDNA) with a AuNP at the 3' end were added to form several layers of AuNP linked structures. Since the permittivity of the medium in which the capacitor is located changed due to the AuNP-linking structures between the gaps of the micro capacitor, the resonant frequency of series LC circuit shifted immediately. The lab-on-a-chip proposed in this study was power supplied by the radio frequency identification (RFID) system. The shift of the LC resonant frequency determined whether the tDNA existed. Thus the detection chip needed no any other internal power supply or expensive instruments for electrical signal measurement. The study provides a simple, fast and convenient platform for biochemical detection.

原文英語
主出版物標題Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
頁面278-282
頁數5
DOIs
出版狀態已發佈 - 2006 十二月 1
事件1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS - Zhuhai, 中国
持續時間: 2006 一月 182006 一月 21

出版系列

名字Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS

其他

其他1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
國家中国
城市Zhuhai
期間06/1/1806/1/21

ASJC Scopus subject areas

  • Biotechnology
  • Materials Science (miscellaneous)

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  • 引用此

    Chien, J. H., Tsai, C. Y., Cheng, Y. T., Yang, C. R., Wang, P. Y., Chang, T. L., Lee, D. S., Chang, C. W., Chou, W. P., Chiang, C. C., Lee, Y. W., & Chen, P. H. (2006). A RFID tag based remote DNA sensing system. 於 Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS (頁 278-282). [4134952] (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS). https://doi.org/10.1109/NEMS.2006.334722