A portable fluorescence resonance energy transfer biosensor for rapid detection of silver ions

Yi Ju Chen, Cheng You Liu, Dong Yu Tsai, Yi Chun Yeh

研究成果: 雜誌貢獻文章

6 引文 斯高帕斯(Scopus)

摘要

In this study, we employed CupR protein in the construct of a fluorescence resonance energy transfer (FRET)-based protein biosensor for the detection of silver ions. We fused the CFP and the YPet through a truncated CupR protein (containing a dimerization helix and a metal binding domain) to create a FRET-based biosensor for the detection of silver ions. Various aqueous media (including tap water and pond water) were examined to determine the matrix effects and agarose gel was used to increase the feasibility of this portable biosensors. The resulting biosensor has demonstrated high sensitivity and selectivity to silver ions.

原文英語
頁(從 - 到)784-788
頁數5
期刊Sensors and Actuators, B: Chemical
259
DOIs
出版狀態已發佈 - 2018 四月 15

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

指紋 深入研究「A portable fluorescence resonance energy transfer biosensor for rapid detection of silver ions」主題。共同形成了獨特的指紋。

  • 引用此