@article{d6e1d6bb5a4b4be69d589add2e193bac,
title = "A new record of Neoperla obliqua Banks, 1930 (Plecoptera: Perlidae) from Mt. Malindang, Mindanao, Philippines and association of life stages using DNA barcodes",
abstract = "The nymph of the perlid Neoperla obliqua Banks, 1913 from Mt. Malindang, Mindanao Island, the Philippines is described and associated with the male and female adults using DNA barcodes. Using pairwise CO1, the nymph was associated with 99.9 ± 0.14% interspecific similarity, while comparison between sexes generated a 0.2% intraspecific divergence between males and females of putative conspecifics. Additionally, the mature ova from female adults are described using scanning electron microscopy (SEM). The distinctive chorionic surface is rugose with longitudinal ridge-like pattern with a bare and flat collar.",
keywords = "DNA barcode, Neoperla, Nymph, Ova, Philippines, Plecoptera",
author = "{Dela Cruz}, {Ian Niel B.} and Nu{\~n}eza, {Olga M.} and Lin, {Chung Ping}",
note = "Funding Information: This study is funded by the Department of Science and Technology (DOST)—ASTHRD Program of the Philippines, and the Ministry of Science and Technology (MOST) of Taiwan (NSC 100-2311-B-029-004-MY3 and MOST 103-2311-B-029-001-MY3 to CPL). Gratuitous permit (No. R102014-05) was granted by the Department of Environment and Natural Resources—Protected Area Management Board Region X, and City Environmental and Natural Resources Office Oroquieta. We thank the Natural Museum of Natural Science, Taichung, Taiwan for SEM support, Ignac Sivec and Peter Zwick for the discussion, the members of the Systematics and Evolutionary Biology Laboratory, National Taiwan Normal University for helpful comments of the manuscript, and the High-Throughput Genome Analysis Core Facility Program for Biotechnology, Taiwan for DNA sequencing. Funding Information: This study is funded by the Department of Science and Technology (DOST)-ASTHRD Program of the Philippines, and the Ministry of Science and Technology (MOST) of Taiwan (NSC 100-2311-B-029-004-MY3 and MOST 103-2311-B-029-001-MY3 to CPL). Gratuitous permit (No. R102014-05) was granted by the Department of Environment and Natural Resources-Protected Area Management Board Region X, and City Environmental and Natural Resources Office Oroquieta. We thank the Natural Museum of Natural Science, Taichung, Taiwan for SEM support, Ignac Sivec and Peter Zwick for the discussion, the members of the Systematics and Evolutionary Biology Laboratory, National Taiwan Normal University for helpful comments of the manuscript, and the High-Throughput Genome Analysis Core Facility Program for Biotechnology, Taiwan for DNA sequencing. Publisher Copyright: Copyright {\textcopyright} 2018 Magnolia Press",
year = "2018",
month = nov,
day = "6",
doi = "10.11646/zootaxa.4514.1.12",
language = "English",
volume = "4514",
pages = "145--150",
journal = "Zootaxa",
issn = "1175-5326",
publisher = "Magnolia Press",
number = "1",
}