A new approach for the detection of a nonfluorescent compound by CE-resonance Raman spectroscopy based on the sweeping-MEKC mode

C. H. Tsai Chih-Hsin; Po Han Cha; Cheng Huang Lin; Ta Chau Chang; Chih Ta Chia

doi:10.1002/elps.200600393

A new approach for the detection of a nonfluorescent compound by CE-resonance Raman spectroscopy based on the sweeping-MEKC mode

C. H. Tsai Chih-Hsin
, Po Han Cha
, Cheng Huang Lin^*
, Ta Chau Chang
, Chih Ta Chia

^*此作品的通信作者

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

20 引文斯高帕斯（Scopus）

摘要

A CE-resonance Raman spectroscopy (CE-RRS) method based on MEKC and sweeping-MEKC modes is described. A nonfluorescent compound, malachite green (MG), and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as model compound and light source, respectively. In order to carry out a quantitative analysis of MG, a monochromator (effective bandwidth, 0.4 nm) was used to collect the specific Raman line at 1616 cm^-1 (N-(φ and C-C stretch, corresponding to 582 nm when the wavelength of the exciting source was 532 nm). As a result, the LOD for MG was 10 ppm, based on the MEKC/RRS mode. This could be improved to 5 ppb when the sweeping-MEKC/RRS mode was applied. Furthermore, with the addition of nano-size silver colloids to the CE buffer the detection limits can be further improved, but the data obtained with surface-enhanced\resonance Raman spectroscopy (SERRS) are less useful for quantitative purposes.

原文	英語
頁（從 - 到）	4688-4693
頁數	6
期刊	Electrophoresis
卷	27
發行號	23
DOIs	https://doi.org/10.1002/elps.200600393
出版狀態	已發佈 - 2006 12月

ASJC Scopus subject areas

分析化學
生物化學
臨床生物化學

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10.1002/elps.200600393

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title = "A new approach for the detection of a nonfluorescent compound by CE-resonance Raman spectroscopy based on the sweeping-MEKC mode",

abstract = "A CE-resonance Raman spectroscopy (CE-RRS) method based on MEKC and sweeping-MEKC modes is described. A nonfluorescent compound, malachite green (MG), and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as model compound and light source, respectively. In order to carry out a quantitative analysis of MG, a monochromator (effective bandwidth, 0.4 nm) was used to collect the specific Raman line at 1616 cm-1 (N-(φ and C-C stretch, corresponding to 582 nm when the wavelength of the exciting source was 532 nm). As a result, the LOD for MG was 10 ppm, based on the MEKC/RRS mode. This could be improved to 5 ppb when the sweeping-MEKC/RRS mode was applied. Furthermore, with the addition of nano-size silver colloids to the CE buffer the detection limits can be further improved, but the data obtained with surface-enhanced\textbackslash{}resonance Raman spectroscopy (SERRS) are less useful for quantitative purposes.",

keywords = "CE, Malachite green, Raman spectroscopy, Surface enhanced-resonance Roman spectroscopy, Sweeping-MEKC",

author = "\{Tsai Chih-Hsin\}, \{C. H.\} and Cha, \{Po Han\} and Lin, \{Cheng Huang\} and Chang, \{Ta Chau\} and Chia, \{Chih Ta\}",

year = "2006",

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doi = "10.1002/elps.200600393",

language = "English",

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T1 - A new approach for the detection of a nonfluorescent compound by CE-resonance Raman spectroscopy based on the sweeping-MEKC mode

AU - Tsai Chih-Hsin, C. H.

AU - Cha, Po Han

AU - Lin, Cheng Huang

AU - Chang, Ta Chau

AU - Chia, Chih Ta

PY - 2006/12

Y1 - 2006/12

N2 - A CE-resonance Raman spectroscopy (CE-RRS) method based on MEKC and sweeping-MEKC modes is described. A nonfluorescent compound, malachite green (MG), and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as model compound and light source, respectively. In order to carry out a quantitative analysis of MG, a monochromator (effective bandwidth, 0.4 nm) was used to collect the specific Raman line at 1616 cm-1 (N-(φ and C-C stretch, corresponding to 582 nm when the wavelength of the exciting source was 532 nm). As a result, the LOD for MG was 10 ppm, based on the MEKC/RRS mode. This could be improved to 5 ppb when the sweeping-MEKC/RRS mode was applied. Furthermore, with the addition of nano-size silver colloids to the CE buffer the detection limits can be further improved, but the data obtained with surface-enhanced\resonance Raman spectroscopy (SERRS) are less useful for quantitative purposes.

AB - A CE-resonance Raman spectroscopy (CE-RRS) method based on MEKC and sweeping-MEKC modes is described. A nonfluorescent compound, malachite green (MG), and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as model compound and light source, respectively. In order to carry out a quantitative analysis of MG, a monochromator (effective bandwidth, 0.4 nm) was used to collect the specific Raman line at 1616 cm-1 (N-(φ and C-C stretch, corresponding to 582 nm when the wavelength of the exciting source was 532 nm). As a result, the LOD for MG was 10 ppm, based on the MEKC/RRS mode. This could be improved to 5 ppb when the sweeping-MEKC/RRS mode was applied. Furthermore, with the addition of nano-size silver colloids to the CE buffer the detection limits can be further improved, but the data obtained with surface-enhanced\resonance Raman spectroscopy (SERRS) are less useful for quantitative purposes.

KW - CE

KW - Malachite green

KW - Raman spectroscopy

KW - Surface enhanced-resonance Roman spectroscopy

KW - Sweeping-MEKC

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SN - 0173-0835

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JO - Electrophoresis

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ER -

A new approach for the detection of a nonfluorescent compound by CE-resonance Raman spectroscopy based on the sweeping-MEKC mode

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