同位素化學標記法搭配質譜技術進行發炎反應動物模式之差異蛋白質體學研究

The precipitation of monosodium urate crystals within joints triggers an acute inflammatory reaction that is the root cause of gout. The inflammation induced by the injection of MSU crystals into the murine air pouch for 1, 3 and 5 h was examined by iTRAQ-based proteomic profiling. The iTRAQ labeled peptides were fractionated by SCX, basic-RP or solution-IEF, following by LC-MS/MS analysis. A total of 951 proteins were quantified from the total combined fractions. Among them, 317 proteins exhibited a differential expression, compared to that of the controls at one time point or more. The majority of the differentially expressed proteins were found in the sample after a 5 h MSU treatment. Western blot revealed that the expression levels of CRAMP and S100A9 were positively correlated with the time-course treated with MSU. Further analysis of GeneGO Pathway enrichment demonstrated that these differentially expressed proteins are primarily related to the immune-related complement system and the tricarboxylic acid cycle.