Yang-Dan-Tang, identified from 15 Chinese herbal formulae, inhibits human lung cancer cell proliferation via cell cycle arrest

Tien Chun Wang, Chih Nan Fang, Chih Chien Shen, Hui Yu Wei, Yui Ping Weng, Jung Yaw Lin, Hsiu Mei Hsieh, Chen Yu Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Lung cancer has long been one of the most deadly forms of cancer. The majority of lung cancers are of the non-small-cell lung cancer (NSCLC) type. Here we used the non-small-cell lung carcinoma cell line A549 to screen 15 different traditional Chinese herbal medicine (CHM) formulae to explore the possible mechanisms of alternative medicine in lung cancer therapy. We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells. Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further characterization. Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death. These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6. This study provides further insight into the possible working mechanism of Yang-Dan-Tang in patients.

Original languageEnglish
Article number276032
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 2012 Jun 20

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Cell Cycle Checkpoints
Lung Neoplasms
Cell Proliferation
Non-Small Cell Lung Carcinoma
Cyclin D3
Cyclin B1
Herbal Medicine
G2 Phase
G1 Phase
Complementary Therapies
Cell Cycle
Flow Cytometry
Cell Death
Western Blotting
Lung
Therapeutics
Neoplasms
A549 Cells

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Yang-Dan-Tang, identified from 15 Chinese herbal formulae, inhibits human lung cancer cell proliferation via cell cycle arrest. / Wang, Tien Chun; Fang, Chih Nan; Shen, Chih Chien; Wei, Hui Yu; Weng, Yui Ping; Lin, Jung Yaw; Hsieh, Hsiu Mei; Lee, Chen Yu.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2012, 276032, 20.06.2012.

Research output: Contribution to journalArticle

Wang, Tien Chun ; Fang, Chih Nan ; Shen, Chih Chien ; Wei, Hui Yu ; Weng, Yui Ping ; Lin, Jung Yaw ; Hsieh, Hsiu Mei ; Lee, Chen Yu. / Yang-Dan-Tang, identified from 15 Chinese herbal formulae, inhibits human lung cancer cell proliferation via cell cycle arrest. In: Evidence-based Complementary and Alternative Medicine. 2012 ; Vol. 2012.
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AU - Wei, Hui Yu

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