Wash-free immunomagnetic detection for serum through magnetic susceptibility reduction

Chin Yih Hong; W. S. Chen; Z. F. Jian; S. Y. Yang; H. E. Horng; L. C. Yang; H. C. Yang

doi:10.1063/1.2536127

Wash-free immunomagnetic detection for serum through magnetic susceptibility reduction

Chin Yih Hong, W. S. Chen, Z. F. Jian, S. Y. Yang, H. E. Horng^*, L. C. Yang, H. C. Yang

^*Corresponding author for this work

Undergraduate Program of Electro-Optical Engineering

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

In this study, a platform for wash-free immunomagnetic detections is discussed. The reagent is a magnetic fluid composed of biofunctionalized magnetic particles dispersed in water. The assay instrument is a mixed-frequency magnetosusceptometer. The mixed-frequency ac magnetic susceptibility ac of the magnetic reagent is reduced, as a result of the formation of magnetic clusters via the association among magnetic nanoparticles and a specific biotarget. The immunoassay detection of proteins in water, such as aqueous c-reactive protein, is characterized and is compared to that in serum. These observations demonstrate the advantages of high convenience, high sensitivity, and high specificity for ac -reduction immunomagnetic detection.

Original language	English
Article number	074105
Journal	Applied Physics Letters
Volume	90
Issue number	7
DOIs	https://doi.org/10.1063/1.2536127
Publication status	Published - 2007

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1063/1.2536127

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abstract = "In this study, a platform for wash-free immunomagnetic detections is discussed. The reagent is a magnetic fluid composed of biofunctionalized magnetic particles dispersed in water. The assay instrument is a mixed-frequency magnetosusceptometer. The mixed-frequency ac magnetic susceptibility ac of the magnetic reagent is reduced, as a result of the formation of magnetic clusters via the association among magnetic nanoparticles and a specific biotarget. The immunoassay detection of proteins in water, such as aqueous c-reactive protein, is characterized and is compared to that in serum. These observations demonstrate the advantages of high convenience, high sensitivity, and high specificity for ac -reduction immunomagnetic detection.",

author = "Hong, {Chin Yih} and Chen, {W. S.} and Jian, {Z. F.} and Yang, {S. Y.} and Horng, {H. E.} and Yang, {L. C.} and Yang, {H. C.}",

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T1 - Wash-free immunomagnetic detection for serum through magnetic susceptibility reduction

AU - Hong, Chin Yih

AU - Chen, W. S.

AU - Jian, Z. F.

AU - Yang, S. Y.

AU - Horng, H. E.

AU - Yang, L. C.

AU - Yang, H. C.

PY - 2007

Y1 - 2007

N2 - In this study, a platform for wash-free immunomagnetic detections is discussed. The reagent is a magnetic fluid composed of biofunctionalized magnetic particles dispersed in water. The assay instrument is a mixed-frequency magnetosusceptometer. The mixed-frequency ac magnetic susceptibility ac of the magnetic reagent is reduced, as a result of the formation of magnetic clusters via the association among magnetic nanoparticles and a specific biotarget. The immunoassay detection of proteins in water, such as aqueous c-reactive protein, is characterized and is compared to that in serum. These observations demonstrate the advantages of high convenience, high sensitivity, and high specificity for ac -reduction immunomagnetic detection.

AB - In this study, a platform for wash-free immunomagnetic detections is discussed. The reagent is a magnetic fluid composed of biofunctionalized magnetic particles dispersed in water. The assay instrument is a mixed-frequency magnetosusceptometer. The mixed-frequency ac magnetic susceptibility ac of the magnetic reagent is reduced, as a result of the formation of magnetic clusters via the association among magnetic nanoparticles and a specific biotarget. The immunoassay detection of proteins in water, such as aqueous c-reactive protein, is characterized and is compared to that in serum. These observations demonstrate the advantages of high convenience, high sensitivity, and high specificity for ac -reduction immunomagnetic detection.

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Wash-free immunomagnetic detection for serum through magnetic susceptibility reduction

Abstract

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