Wash-free, antibody-assisted magnetoreduction assays of orchid viruses

Shieh-Yueh Yang; Z. F. Jian; J. J. Chieh; Herng-Er Horng; Hong-Chang Yang; I. J. Huang; Chin Yih Hong

doi:10.1016/j.jviromet.2008.01.019

Wash-free, antibody-assisted magnetoreduction assays of orchid viruses

Shieh-Yueh Yang, Z. F. Jian, J. J. Chieh, Herng-Er Horng^*, Hong-Chang Yang, I. J. Huang, Chin Yih Hong

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

This study demonstrates the feasibility of wash-free magnetoreduction assays (MRA) of orchid viruses. A magnetic reagent, consisting of magnetic beads coated with antibodies and dispersed in water, was synthesized. By using a mixed-frequency alternative-current (ac) magnetosusceptometer, differences in the magnetic susceptibilities of the magnetic reagent before and after the addition of orchid solutions were measured. The results show significant advantages for MRA of orchid viruses.

Original language	English
Pages (from-to)	334-337
Number of pages	4
Journal	Journal of Virological Methods
Volume	149
Issue number	2
DOIs	https://doi.org/10.1016/j.jviromet.2008.01.019
Publication status	Published - 2008 May

Keywords

Assay
Magnetic beads
Magnetoreduction
Orchid virus

ASJC Scopus subject areas

Virology

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10.1016/j.jviromet.2008.01.019

Cite this

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title = "Wash-free, antibody-assisted magnetoreduction assays of orchid viruses",

abstract = "This study demonstrates the feasibility of wash-free magnetoreduction assays (MRA) of orchid viruses. A magnetic reagent, consisting of magnetic beads coated with antibodies and dispersed in water, was synthesized. By using a mixed-frequency alternative-current (ac) magnetosusceptometer, differences in the magnetic susceptibilities of the magnetic reagent before and after the addition of orchid solutions were measured. The results show significant advantages for MRA of orchid viruses.",

keywords = "Assay, Magnetic beads, Magnetoreduction, Orchid virus",

author = "Shieh-Yueh Yang and Jian, {Z. F.} and Chieh, {J. J.} and Herng-Er Horng and Hong-Chang Yang and Huang, {I. J.} and Hong, {Chin Yih}",

note = "Funding Information: This work was supported by the National Science Council of Taiwan under Grant Nos. 95-2120-M-003-001, 95-2112-M-003-017-MY2, 96-2112-M-002-025, 96-2752-M-002-016-PAE, and 96-2628-M-003-002.",

year = "2008",

month = may,

doi = "10.1016/j.jviromet.2008.01.019",

language = "English",

volume = "149",

pages = "334--337",

journal = "Journal of Virological Methods",

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T1 - Wash-free, antibody-assisted magnetoreduction assays of orchid viruses

AU - Yang, Shieh-Yueh

AU - Jian, Z. F.

AU - Chieh, J. J.

AU - Horng, Herng-Er

AU - Yang, Hong-Chang

AU - Huang, I. J.

AU - Hong, Chin Yih

N1 - Funding Information: This work was supported by the National Science Council of Taiwan under Grant Nos. 95-2120-M-003-001, 95-2112-M-003-017-MY2, 96-2112-M-002-025, 96-2752-M-002-016-PAE, and 96-2628-M-003-002.

PY - 2008/5

Y1 - 2008/5

N2 - This study demonstrates the feasibility of wash-free magnetoreduction assays (MRA) of orchid viruses. A magnetic reagent, consisting of magnetic beads coated with antibodies and dispersed in water, was synthesized. By using a mixed-frequency alternative-current (ac) magnetosusceptometer, differences in the magnetic susceptibilities of the magnetic reagent before and after the addition of orchid solutions were measured. The results show significant advantages for MRA of orchid viruses.

AB - This study demonstrates the feasibility of wash-free magnetoreduction assays (MRA) of orchid viruses. A magnetic reagent, consisting of magnetic beads coated with antibodies and dispersed in water, was synthesized. By using a mixed-frequency alternative-current (ac) magnetosusceptometer, differences in the magnetic susceptibilities of the magnetic reagent before and after the addition of orchid solutions were measured. The results show significant advantages for MRA of orchid viruses.

KW - Assay

KW - Magnetic beads

KW - Magnetoreduction

KW - Orchid virus

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AN - SCOPUS:41349116711

SN - 0166-0934

VL - 149

SP - 334

EP - 337

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 2

ER -

Wash-free, antibody-assisted magnetoreduction assays of orchid viruses

Abstract

Keywords

ASJC Scopus subject areas

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