Wash-free, antibody-assisted magnetoreduction assays of orchid viruses

S. Y. Yang, Z. F. Jian, J. J. Chieh, H. E. Horng, H. C. Yang, I. J. Huang, Chin Yih Hong

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

This study demonstrates the feasibility of wash-free magnetoreduction assays (MRA) of orchid viruses. A magnetic reagent, consisting of magnetic beads coated with antibodies and dispersed in water, was synthesized. By using a mixed-frequency alternative-current (ac) magnetosusceptometer, differences in the magnetic susceptibilities of the magnetic reagent before and after the addition of orchid solutions were measured. The results show significant advantages for MRA of orchid viruses.

Original languageEnglish
Pages (from-to)334-337
Number of pages4
JournalJournal of Virological Methods
Volume149
Issue number2
DOIs
Publication statusPublished - 2008 May 1

Keywords

  • Assay
  • Magnetic beads
  • Magnetoreduction
  • Orchid virus

ASJC Scopus subject areas

  • Virology

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