Vitreous Levels of Reactive Oxygen Species in Proliferative Diabetic Retinopathy

Po Ting Yeh, Chung May Yang, Jen Shang Huang, Chiang-Ting Chien, Chang Hao Yang, Yi Hui Chiang, Yung Feng Shih

Research output: Contribution to journalArticle

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Abstract

Objective: To investigate vitreous levels of reactive oxygen species (ROS) in patients with proliferative diabetic retinopathy (PDR) and analyze ROS levels among different groups of PDR patients. Design: Retrospective case-control study. Participants: Thirty-nine eyes of 39 patients with PDR and 16 eyes of 16 non-PDR patients (control group) that underwent primary vitrectomy for complications of PDR and other conditions (control group), with a follow-up time ≥ 12 months. Methods: Proliferative diabetic retinopathy patients were classified into 3 groups according to the extent of fibrovascular proliferation: (1) no or focal adhesions at ≤3 sites (n = 17); (2) ≥1 broad adhesions or vitreous-retinal adhesions around disc, macula, and arcade (n = 12); and (3) vitreous-retinal attachment extending to the periphery or no posterior vitreous detachment with or without retinal detachment (RD) (n = 10). The control group (n = 16) contained non-PDR patients. Vitreous samples were obtained during measurement of vitrectomy and vitreous levels of ROS by luminol-enhanced chemiluminescence assay. Main Outcome Measures: Reactive oxygen species levels were recorded as mean (± standard deviation) chemiluminescence counts per 10 seconds. Correlations of vitreous levels of ROS among the 3 PDR groups and anatomic prognosis were evaluated. Multiple linear regression analysis of selective potential risk factors was performed to investigate the main determinants of ROS levels. Results: Vitreous ROS levels were significantly higher in patients with PDR (125.76±351.72 chemiluminescence counts per 10 seconds) than in control patients (0.37±0.72 chemiluminescence counts per 10 seconds; P<0.0001). Reactive oxygen species levels were 1.86±1.63 (group 1), 24.47±22.68 (group 2), and 457.94±597.01 (group 3); the difference among groups was significant (P = 0.001). Regression analysis indicated that only patient grouping (according to the severity of fibrovascular proliferation) had a strong dependent association with ROS levels (P = 0.001). Final anatomic results revealed that recurrent untreatable RD occurred in 3 patients of group 3, who also had the highest ROS levels. Conclusions: Reactive oxygen species levels were significantly elevated in the vitreous fluid of PDR patients, and patients with a more advanced clinical PDR appearance had higher ROS levels. These findings suggest an association between ROS and the pathogenesis of PDR. Reactive oxygen species might be correlated with PDR severity.

Original languageEnglish
JournalOphthalmology
Volume115
Issue number4
DOIs
Publication statusPublished - 2008 Jan 1

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Diabetic Retinopathy
Reactive Oxygen Species
Luminescence
Vitrectomy
Retinal Detachment
Control Groups
Regression Analysis
Vitreous Detachment
Luminol
Focal Adhesions
Case-Control Studies
Linear Models

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Yeh, P. T., Yang, C. M., Huang, J. S., Chien, C-T., Yang, C. H., Chiang, Y. H., & Shih, Y. F. (2008). Vitreous Levels of Reactive Oxygen Species in Proliferative Diabetic Retinopathy. Ophthalmology, 115(4). https://doi.org/10.1016/j.ophtha.2007.05.041

Vitreous Levels of Reactive Oxygen Species in Proliferative Diabetic Retinopathy. / Yeh, Po Ting; Yang, Chung May; Huang, Jen Shang; Chien, Chiang-Ting; Yang, Chang Hao; Chiang, Yi Hui; Shih, Yung Feng.

In: Ophthalmology, Vol. 115, No. 4, 01.01.2008.

Research output: Contribution to journalArticle

Yeh, Po Ting ; Yang, Chung May ; Huang, Jen Shang ; Chien, Chiang-Ting ; Yang, Chang Hao ; Chiang, Yi Hui ; Shih, Yung Feng. / Vitreous Levels of Reactive Oxygen Species in Proliferative Diabetic Retinopathy. In: Ophthalmology. 2008 ; Vol. 115, No. 4.
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abstract = "Objective: To investigate vitreous levels of reactive oxygen species (ROS) in patients with proliferative diabetic retinopathy (PDR) and analyze ROS levels among different groups of PDR patients. Design: Retrospective case-control study. Participants: Thirty-nine eyes of 39 patients with PDR and 16 eyes of 16 non-PDR patients (control group) that underwent primary vitrectomy for complications of PDR and other conditions (control group), with a follow-up time ≥ 12 months. Methods: Proliferative diabetic retinopathy patients were classified into 3 groups according to the extent of fibrovascular proliferation: (1) no or focal adhesions at ≤3 sites (n = 17); (2) ≥1 broad adhesions or vitreous-retinal adhesions around disc, macula, and arcade (n = 12); and (3) vitreous-retinal attachment extending to the periphery or no posterior vitreous detachment with or without retinal detachment (RD) (n = 10). The control group (n = 16) contained non-PDR patients. Vitreous samples were obtained during measurement of vitrectomy and vitreous levels of ROS by luminol-enhanced chemiluminescence assay. Main Outcome Measures: Reactive oxygen species levels were recorded as mean (± standard deviation) chemiluminescence counts per 10 seconds. Correlations of vitreous levels of ROS among the 3 PDR groups and anatomic prognosis were evaluated. Multiple linear regression analysis of selective potential risk factors was performed to investigate the main determinants of ROS levels. Results: Vitreous ROS levels were significantly higher in patients with PDR (125.76±351.72 chemiluminescence counts per 10 seconds) than in control patients (0.37±0.72 chemiluminescence counts per 10 seconds; P<0.0001). Reactive oxygen species levels were 1.86±1.63 (group 1), 24.47±22.68 (group 2), and 457.94±597.01 (group 3); the difference among groups was significant (P = 0.001). Regression analysis indicated that only patient grouping (according to the severity of fibrovascular proliferation) had a strong dependent association with ROS levels (P = 0.001). Final anatomic results revealed that recurrent untreatable RD occurred in 3 patients of group 3, who also had the highest ROS levels. Conclusions: Reactive oxygen species levels were significantly elevated in the vitreous fluid of PDR patients, and patients with a more advanced clinical PDR appearance had higher ROS levels. These findings suggest an association between ROS and the pathogenesis of PDR. Reactive oxygen species might be correlated with PDR severity.",
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AU - Yang, Chang Hao

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AU - Shih, Yung Feng

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N2 - Objective: To investigate vitreous levels of reactive oxygen species (ROS) in patients with proliferative diabetic retinopathy (PDR) and analyze ROS levels among different groups of PDR patients. Design: Retrospective case-control study. Participants: Thirty-nine eyes of 39 patients with PDR and 16 eyes of 16 non-PDR patients (control group) that underwent primary vitrectomy for complications of PDR and other conditions (control group), with a follow-up time ≥ 12 months. Methods: Proliferative diabetic retinopathy patients were classified into 3 groups according to the extent of fibrovascular proliferation: (1) no or focal adhesions at ≤3 sites (n = 17); (2) ≥1 broad adhesions or vitreous-retinal adhesions around disc, macula, and arcade (n = 12); and (3) vitreous-retinal attachment extending to the periphery or no posterior vitreous detachment with or without retinal detachment (RD) (n = 10). The control group (n = 16) contained non-PDR patients. Vitreous samples were obtained during measurement of vitrectomy and vitreous levels of ROS by luminol-enhanced chemiluminescence assay. Main Outcome Measures: Reactive oxygen species levels were recorded as mean (± standard deviation) chemiluminescence counts per 10 seconds. Correlations of vitreous levels of ROS among the 3 PDR groups and anatomic prognosis were evaluated. Multiple linear regression analysis of selective potential risk factors was performed to investigate the main determinants of ROS levels. Results: Vitreous ROS levels were significantly higher in patients with PDR (125.76±351.72 chemiluminescence counts per 10 seconds) than in control patients (0.37±0.72 chemiluminescence counts per 10 seconds; P<0.0001). Reactive oxygen species levels were 1.86±1.63 (group 1), 24.47±22.68 (group 2), and 457.94±597.01 (group 3); the difference among groups was significant (P = 0.001). Regression analysis indicated that only patient grouping (according to the severity of fibrovascular proliferation) had a strong dependent association with ROS levels (P = 0.001). Final anatomic results revealed that recurrent untreatable RD occurred in 3 patients of group 3, who also had the highest ROS levels. Conclusions: Reactive oxygen species levels were significantly elevated in the vitreous fluid of PDR patients, and patients with a more advanced clinical PDR appearance had higher ROS levels. These findings suggest an association between ROS and the pathogenesis of PDR. Reactive oxygen species might be correlated with PDR severity.

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