Violet light emitting diode-induced fluorescence detection combined with on-line sample concentration techniques for use in capillary electrophoresis

Chih Hsin Tsai; Hsuan Ming Huang; Cheng Huang Lin

doi:10.1002/elps.200305505

Violet light emitting diode-induced fluorescence detection combined with on-line sample concentration techniques for use in capillary electrophoresis

Chih Hsin Tsai, Hsuan Ming Huang, Cheng Huang Lin^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

The first application of a violet light-emitting diode (LED) for fluorescence detection in capillary electrophoresis (CE) is described. The utility of violet LED (peak emission wavelength at 410 nm, ∼2 mW) for fluorescence detection is demonstrated by examining reserpine and dopamine-labeled NDA (naphthalene-2,3-dicarboxaldehyde), respectively. The detection limit for reserpine was determined to be 2.5 × 10^-6 M by normal micellar electrokinetic capillary chromatography (MEKC) and this was improved to 2.0 × 10^-9 M and 2.0 × 10^-10 M when sweeping-MEKC and cation-selective exhaustive injection (CSEI)-sweep-MEKC techniques were applied, respectively. In addition, the detection limit of NDA-labeled dopamine was determined to be 6.3 × 10^-6 M by means of normal MEKC and this was improved to 3.0 × 10^-8 M when the sweeping-MEKC mode was applied.

Original language	English
Pages (from-to)	3083-3088
Number of pages	6
Journal	Electrophoresis
Volume	24
Issue number	17
DOIs	https://doi.org/10.1002/elps.200305505
Publication status	Published - 2003 Sept

Keywords

Capillary electrophoresis
Dopamine
Reserpine
Violet ligth-emitting diode

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/elps.200305505

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title = "Violet light emitting diode-induced fluorescence detection combined with on-line sample concentration techniques for use in capillary electrophoresis",

abstract = "The first application of a violet light-emitting diode (LED) for fluorescence detection in capillary electrophoresis (CE) is described. The utility of violet LED (peak emission wavelength at 410 nm, ∼2 mW) for fluorescence detection is demonstrated by examining reserpine and dopamine-labeled NDA (naphthalene-2,3-dicarboxaldehyde), respectively. The detection limit for reserpine was determined to be 2.5 × 10-6 M by normal micellar electrokinetic capillary chromatography (MEKC) and this was improved to 2.0 × 10-9 M and 2.0 × 10-10 M when sweeping-MEKC and cation-selective exhaustive injection (CSEI)-sweep-MEKC techniques were applied, respectively. In addition, the detection limit of NDA-labeled dopamine was determined to be 6.3 × 10-6 M by means of normal MEKC and this was improved to 3.0 × 10-8 M when the sweeping-MEKC mode was applied.",

keywords = "Capillary electrophoresis, Dopamine, Reserpine, Violet ligth-emitting diode",

author = "Tsai, {Chih Hsin} and Huang, {Hsuan Ming} and Lin, {Cheng Huang}",

year = "2003",

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doi = "10.1002/elps.200305505",

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volume = "24",

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AU - Tsai, Chih Hsin

AU - Huang, Hsuan Ming

AU - Lin, Cheng Huang

PY - 2003/9

Y1 - 2003/9

N2 - The first application of a violet light-emitting diode (LED) for fluorescence detection in capillary electrophoresis (CE) is described. The utility of violet LED (peak emission wavelength at 410 nm, ∼2 mW) for fluorescence detection is demonstrated by examining reserpine and dopamine-labeled NDA (naphthalene-2,3-dicarboxaldehyde), respectively. The detection limit for reserpine was determined to be 2.5 × 10-6 M by normal micellar electrokinetic capillary chromatography (MEKC) and this was improved to 2.0 × 10-9 M and 2.0 × 10-10 M when sweeping-MEKC and cation-selective exhaustive injection (CSEI)-sweep-MEKC techniques were applied, respectively. In addition, the detection limit of NDA-labeled dopamine was determined to be 6.3 × 10-6 M by means of normal MEKC and this was improved to 3.0 × 10-8 M when the sweeping-MEKC mode was applied.

AB - The first application of a violet light-emitting diode (LED) for fluorescence detection in capillary electrophoresis (CE) is described. The utility of violet LED (peak emission wavelength at 410 nm, ∼2 mW) for fluorescence detection is demonstrated by examining reserpine and dopamine-labeled NDA (naphthalene-2,3-dicarboxaldehyde), respectively. The detection limit for reserpine was determined to be 2.5 × 10-6 M by normal micellar electrokinetic capillary chromatography (MEKC) and this was improved to 2.0 × 10-9 M and 2.0 × 10-10 M when sweeping-MEKC and cation-selective exhaustive injection (CSEI)-sweep-MEKC techniques were applied, respectively. In addition, the detection limit of NDA-labeled dopamine was determined to be 6.3 × 10-6 M by means of normal MEKC and this was improved to 3.0 × 10-8 M when the sweeping-MEKC mode was applied.

KW - Capillary electrophoresis

KW - Dopamine

KW - Reserpine

KW - Violet ligth-emitting diode

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JO - Electrophoresis

JF - Electrophoresis

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ER -

Violet light emitting diode-induced fluorescence detection combined with on-line sample concentration techniques for use in capillary electrophoresis

Abstract

Keywords

ASJC Scopus subject areas

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