Violet light emitting diode-induced fluorescence detection combined with on-line sample concentration techniques for use in capillary electrophoresis

Chih Hsin Tsai, Hsuan Ming Huang, Cheng Huang Lin

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The first application of a violet light-emitting diode (LED) for fluorescence detection in capillary electrophoresis (CE) is described. The utility of violet LED (peak emission wavelength at 410 nm, ∼2 mW) for fluorescence detection is demonstrated by examining reserpine and dopamine-labeled NDA (naphthalene-2,3-dicarboxaldehyde), respectively. The detection limit for reserpine was determined to be 2.5 × 10-6 M by normal micellar electrokinetic capillary chromatography (MEKC) and this was improved to 2.0 × 10-9 M and 2.0 × 10-10 M when sweeping-MEKC and cation-selective exhaustive injection (CSEI)-sweep-MEKC techniques were applied, respectively. In addition, the detection limit of NDA-labeled dopamine was determined to be 6.3 × 10-6 M by means of normal MEKC and this was improved to 3.0 × 10-8 M when the sweeping-MEKC mode was applied.

Original languageEnglish
Pages (from-to)3083-3088
Number of pages6
JournalElectrophoresis
Volume24
Issue number17
DOIs
Publication statusPublished - 2003 Sep 1

    Fingerprint

Keywords

  • Capillary electrophoresis
  • Dopamine
  • Reserpine
  • Violet ligth-emitting diode

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this