Using bio-functionalized magnetic nanoparticles and dynamic nuclear magnetic resonance to characterize the time-dependent spin-spin relaxation time for sensitive bio-detection

Shu Hsien Liao, Kuen Lin Chen, Chun Ming Wang, Jen Jie Chieh, Herng Er Horng, Li Min Wang, C. H. Wu, Hong Chang Yang

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    In this work, we report the use of bio-functionalized magnetic nanoparticles (BMNs) and dynamic magnetic resonance (DMR) to characterize the time-dependent spin-spin relaxation time for sensitive bio-detection. The biomarkers are the human C-reactive protein (CRP) while the BMNs are the anti-CRP bound onto dextran-coated Fe3O4 particles labeled as Fe3O4-antiCRP. It was found the time-dependent spin-spin relaxation time, T2, of protons decreases as time evolves. Additionally, the ΔT2 of of protons in BMNs increases as the concentration of CRP increases. We attribute these to the formation of the magnetic clusters that deteriorate the field homogeneity of nearby protons. A sensitivity better than 0.1 μg/mL for assaying CRP is achieved, which is much higher than that required by the clinical criteria (0.5 mg/dL). The present MR-detection platform shows promise for further use in detecting tumors, viruses, and proteins.

    Original languageEnglish
    Pages (from-to)21409-21417
    Number of pages9
    JournalSensors (Switzerland)
    Volume14
    Issue number11
    DOIs
    Publication statusPublished - 2014 Nov 12

    Keywords

    • CRP
    • NMR
    • Spin-spin relaxation time

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry
    • Atomic and Molecular Physics, and Optics
    • Instrumentation
    • Electrical and Electronic Engineering

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