Using bio-functionalized magnetic nanoparticles and dynamic nuclear magnetic resonance to characterize the time-dependent spin-spin relaxation time for sensitive bio-detection

Shu Hsien Liao, Kuen Lin Chen, Chun Ming Wang, Jen Jie Chieh, Herng Er Horng, Li Min Wang, C. H. Wu, Hong Chang Yang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In this work, we report the use of bio-functionalized magnetic nanoparticles (BMNs) and dynamic magnetic resonance (DMR) to characterize the time-dependent spin-spin relaxation time for sensitive bio-detection. The biomarkers are the human C-reactive protein (CRP) while the BMNs are the anti-CRP bound onto dextran-coated Fe3O4 particles labeled as Fe3O4-antiCRP. It was found the time-dependent spin-spin relaxation time, T2, of protons decreases as time evolves. Additionally, the ΔT2 of of protons in BMNs increases as the concentration of CRP increases. We attribute these to the formation of the magnetic clusters that deteriorate the field homogeneity of nearby protons. A sensitivity better than 0.1 μg/mL for assaying CRP is achieved, which is much higher than that required by the clinical criteria (0.5 mg/dL). The present MR-detection platform shows promise for further use in detecting tumors, viruses, and proteins.

Original languageEnglish
Pages (from-to)21409-21417
Number of pages9
JournalSensors (Switzerland)
Volume14
Issue number11
DOIs
Publication statusPublished - 2014 Nov 12

Keywords

  • CRP
  • NMR
  • Spin-spin relaxation time

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Electrical and Electronic Engineering

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