Use of immunomagnetic reduction for C-reactive protein assay in clinical samples

Chien Hsi Chang, Zhi Xian Lai, Hsiu Li Lin, Che Chuan Yang, Hsin Hsien Chen, Shieh Yueh Yang, Herng Er Horng, Chin Yih Hong, Hong Chang Yang, Hsiu Chen Lin

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    Background: Magnetic nanoparticles biofunctionalized with antibodies are able to recognize and bind to the corresponding antigens. In this work, anti-C-reactive protein (CRP) antibody was covalently conjugated onto the surface of magnetic nanoparticles to label CRP specifically in serum. Methods: The level of serum CRP was detected by immunomagnetic reduction (IMR) assay, which identifies the changes in the magnetic signal representing the level of interaction between antibody-conjugated magnetic nanoparticles and CRP proteins. To investigate the feasibility of IMR for clinical application, pure CRP solutions and 40 human serum samples were tested for IMR detection of CRP to characterize sensitivity, specificity, and interference. Results: In comparison with the immunoturbidimetry assay, the results of the IMR assay indicated higher sensitivity and had a high correlation with those of the current immunoturbidimetry assay. Conclusion: We have developed a novel and promising way to assay CRP in human serum using immunomagnetic reduction in clinical diagnosis.

    Original languageEnglish
    Pages (from-to)4335-4340
    Number of pages6
    JournalInternational Journal of Nanomedicine
    Volume7
    DOIs
    Publication statusPublished - 2012

    Keywords

    • C-reactive protein
    • Immunomagnetic reduction
    • Magnetic nanoparticles

    ASJC Scopus subject areas

    • Biophysics
    • Bioengineering
    • Biomaterials
    • Pharmaceutical Science
    • Drug Discovery
    • Organic Chemistry

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