Unilateral stimulation of the lateral division of the dorsal telencephalon induces synaptic plasticity in the bilateral medial division of zebrafish

Yao Ju Wu, Yu Lan Chen, Tso Hao Tang, Ming Chong Ng, Tamara G. Amstislavskaya, Maria A. Tikhonova, Yi Ling Yang, Kwok Tung Lu

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Abstract

This study was aimed to evaluate the synaptic plasticity in projections from the dorsal lateral region (Dl) to the bilateral dorsal medial region (Dm) of the zebrafish telencephalon. The results showed that unilateral electrical stimulation of the Dl evokes a negative field potential (FP) in both the contralateral and ipsilateral side of the Dm. We tested synaptic plasticity, including high-frequency stimulation-induced LTP (HFS-LTP) and low-frequency stimulation-induced LTD (LFS-LTD). We demonstrated that HFS-induced bilateral LTP is NMDAR-dependent by the application of an NMDAR antagonist, DL-AP5 (30 μM, suprafused for 10 min), which blocked the HFS-induced LTP in both the contralateral and ipsilateral Dm. In addition, LTP was restored after DL-AP5 was washed out by continuous aCSF suprafusion. These results suggested that the potentiation is NMDAR-dependent. Either LFS (1 Hz for 20 min) or applying the mGluR agonist, DHPG (40 μM, suprafused for 10 min) successfully induced bilateral LTD for at least 1 h. Furthermore, both the contralateral fEPSP and LTP vanished after ablation of the anterior commissure. In conclusion, the results of the present study suggested that the projection between the Dl and contralateral Dm in the telencephalon of zebrafish is via the anterior commissure and possesses synaptic plasticity.

Original languageEnglish
Article number9096
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Telencephalon
Neuronal Plasticity
Zebrafish
Electric Stimulation

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Unilateral stimulation of the lateral division of the dorsal telencephalon induces synaptic plasticity in the bilateral medial division of zebrafish. / Wu, Yao Ju; Chen, Yu Lan; Tang, Tso Hao; Ng, Ming Chong; Amstislavskaya, Tamara G.; Tikhonova, Maria A.; Yang, Yi Ling; Lu, Kwok Tung.

In: Scientific Reports, Vol. 7, No. 1, 9096, 01.12.2017.

Research output: Contribution to journalArticle

Wu, Yao Ju ; Chen, Yu Lan ; Tang, Tso Hao ; Ng, Ming Chong ; Amstislavskaya, Tamara G. ; Tikhonova, Maria A. ; Yang, Yi Ling ; Lu, Kwok Tung. / Unilateral stimulation of the lateral division of the dorsal telencephalon induces synaptic plasticity in the bilateral medial division of zebrafish. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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