Abstract
The interactions between pyrroloquinoline quinone (PQQ) and β-nicotinamide adenine dinucleotide (NAD(H)) characterized by spectral and voltammetric means are reported in this paper. PQQ exists with six major acid-base derivatives in aqueous solutions; the derivative predominating at pH 3, denoted H2Q-, was characterized to be responsible for the electrochemical and photochemical activities of PQQ, such as for the photooxidation of NADH. In contrast, the derivatives predominating at pH > 5, such as HQ2- and Q3-, are responsible for the interaction with NAD+; the equilibrium constant was estimated to be ∼106. Although the basic derivatives are less active in emission compared to H2Q-, incorporating NAD+ can enhance their activity in this respect. The titrations of NAD+ with HQ2- and Q3- confirm the complexation reactivity of PQQ to NAD+, and suggest that the reaction sites of PQQ for NAD+ are the same as for Ca2+ ions. Thanks to those unique properties, PQQ can photochemically sense glucose and ethanol as NAD+ and the respective dehydrogenases are incorporated.
Original language | English |
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Pages (from-to) | 141-149 |
Number of pages | 9 |
Journal | Journal of Electroanalytical Chemistry |
Volume | 606 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2007 Aug 15 |
Keywords
- Biosensing
- Pyrroloquinoline quinone
- β-Nicotinamide adenine dinucleotide
ASJC Scopus subject areas
- Analytical Chemistry
- Chemical Engineering(all)
- Electrochemistry