Abstract
The interactions between pyrroloquinoline quinone (PQQ) and β-nicotinamide adenine dinucleotide (NAD(H)) characterized by spectral and voltammetric means are reported in this paper. PQQ exists with six major acid-base derivatives in aqueous solutions; the derivative predominating at pH 3, denoted H2Q-, was characterized to be responsible for the electrochemical and photochemical activities of PQQ, such as for the photooxidation of NADH. In contrast, the derivatives predominating at pH > 5, such as HQ2- and Q3-, are responsible for the interaction with NAD+; the equilibrium constant was estimated to be ∼106. Although the basic derivatives are less active in emission compared to H2Q-, incorporating NAD+ can enhance their activity in this respect. The titrations of NAD+ with HQ2- and Q3- confirm the complexation reactivity of PQQ to NAD+, and suggest that the reaction sites of PQQ for NAD+ are the same as for Ca2+ ions. Thanks to those unique properties, PQQ can photochemically sense glucose and ethanol as NAD+ and the respective dehydrogenases are incorporated.
| Original language | English |
|---|---|
| Pages (from-to) | 141-149 |
| Number of pages | 9 |
| Journal | Journal of Electroanalytical Chemistry |
| Volume | 606 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2007 Aug 15 |
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Keywords
- Biosensing
- Pyrroloquinoline quinone
- β-Nicotinamide adenine dinucleotide
ASJC Scopus subject areas
- Analytical Chemistry
- Chemical Engineering(all)
- Electrochemistry
Cite this
Unexplored interactions between pyrroloquinoline quinone and β-nicotinamide adenine dinucleotide. / Jao, Hsi Jung; Tsai, Pei Yi; Wang, Chong Mou.
In: Journal of Electroanalytical Chemistry, Vol. 606, No. 2, 15.08.2007, p. 141-149.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Unexplored interactions between pyrroloquinoline quinone and β-nicotinamide adenine dinucleotide
AU - Jao, Hsi Jung
AU - Tsai, Pei Yi
AU - Wang, Chong Mou
PY - 2007/8/15
Y1 - 2007/8/15
N2 - The interactions between pyrroloquinoline quinone (PQQ) and β-nicotinamide adenine dinucleotide (NAD(H)) characterized by spectral and voltammetric means are reported in this paper. PQQ exists with six major acid-base derivatives in aqueous solutions; the derivative predominating at pH 3, denoted H2Q-, was characterized to be responsible for the electrochemical and photochemical activities of PQQ, such as for the photooxidation of NADH. In contrast, the derivatives predominating at pH > 5, such as HQ2- and Q3-, are responsible for the interaction with NAD+; the equilibrium constant was estimated to be ∼106. Although the basic derivatives are less active in emission compared to H2Q-, incorporating NAD+ can enhance their activity in this respect. The titrations of NAD+ with HQ2- and Q3- confirm the complexation reactivity of PQQ to NAD+, and suggest that the reaction sites of PQQ for NAD+ are the same as for Ca2+ ions. Thanks to those unique properties, PQQ can photochemically sense glucose and ethanol as NAD+ and the respective dehydrogenases are incorporated.
AB - The interactions between pyrroloquinoline quinone (PQQ) and β-nicotinamide adenine dinucleotide (NAD(H)) characterized by spectral and voltammetric means are reported in this paper. PQQ exists with six major acid-base derivatives in aqueous solutions; the derivative predominating at pH 3, denoted H2Q-, was characterized to be responsible for the electrochemical and photochemical activities of PQQ, such as for the photooxidation of NADH. In contrast, the derivatives predominating at pH > 5, such as HQ2- and Q3-, are responsible for the interaction with NAD+; the equilibrium constant was estimated to be ∼106. Although the basic derivatives are less active in emission compared to H2Q-, incorporating NAD+ can enhance their activity in this respect. The titrations of NAD+ with HQ2- and Q3- confirm the complexation reactivity of PQQ to NAD+, and suggest that the reaction sites of PQQ for NAD+ are the same as for Ca2+ ions. Thanks to those unique properties, PQQ can photochemically sense glucose and ethanol as NAD+ and the respective dehydrogenases are incorporated.
KW - Biosensing
KW - Pyrroloquinoline quinone
KW - β-Nicotinamide adenine dinucleotide
UR - http://www.scopus.com/inward/record.url?scp=34447513960&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34447513960&partnerID=8YFLogxK
U2 - 10.1016/j.jelechem.2007.05.019
DO - 10.1016/j.jelechem.2007.05.019
M3 - Article
AN - SCOPUS:34447513960
VL - 606
SP - 141
EP - 149
JO - Journal of Electroanalytical Chemistry
JF - Journal of Electroanalytical Chemistry
SN - 1572-6657
IS - 2
ER -