@inproceedings{280e47860a4340888d8a282e898dfe0a,
title = "Ultrasensitive protein detection using an Au/ZnO SPR-based biosensor",
abstract = "In this study, we demonstrated that the Au/ZnO nanocomposite films could be effectively used to enhance the performance of SPR for the detection of tumor marker. CA15.3 tumor marker for breast cancer was chosen as a model analyte. We analyzed experimentally intensity response to the samples that had different concentrations (0.0125 U/ml to 160 U/ml) in pleural fluid to evaluate the detection capability of the Au/ZnO SPR-based biosensor. As a result, it was found that the linear range extended from 0.025 to 40 U/ml with correlation coefficient of R = 0.9531 and the limit of detection at S/N of 3:1 reached 0.025U/ml. Compared with the shift degree of SPR intensity by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers increased at least 2 fold more than that on the Au/Cr layers. In addition, we determined that the Au/ZnO layers had a detection limit 4 times lower than the Au/Cr layers that were widespread use of the sensing interfaces on SPR. In conclusion, the use of Au/ZnO films greatly enhanced the SPR signal after carrying out bimolecular interactions and presented high sensitivity and reproducibility.",
keywords = "Au/ZnO, Carbohydrate antigen 15.3, Tumor marker, Ultrasensitive",
author = "Chang, {C. C.} and Lin, {S. H.} and Chiu, {N. F.} and Lin, {C. W.}",
year = "2009",
language = "English",
isbn = "9781439817834",
series = "Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009",
pages = "172--175",
booktitle = "Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009",
note = "Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 ; Conference date: 03-05-2009 Through 07-05-2009",
}
