Abstract
In this study, we demonstrated that the Au/ZnO nanocomposite films could be effectively used to enhance the performance of SPR for the detection of tumor marker. CA15.3 tumor marker for breast cancer was chosen as a model analyte. We analyzed experimentally intensity response to the samples that had different concentrations (0.0125 U/ml to 160 U/ml) in pleural fluid to evaluate the detection capability of the Au/ZnO SPR-based biosensor. As a result, it was found that the linear range extended from 0.025 to 40 U/ml with correlation coefficient of R = 0.9531 and the limit of detection at S/N of 3:1 reached 0.025U/ml. Compared with the shift degree of SPR intensity by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers increased at least 2 fold more than that on the Au/Cr layers. In addition, we determined that the Au/ZnO layers had a detection limit 4 times lower than the Au/Cr layers that were widespread use of the sensing interfaces on SPR. In conclusion, the use of Au/ZnO films greatly enhanced the SPR signal after carrying out bimolecular interactions and presented high sensitivity and reproducibility.
| Original language | English |
|---|---|
| Title of host publication | Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 |
| Pages | 172-175 |
| Number of pages | 4 |
| Volume | 2 |
| Publication status | Published - 2009 Dec 1 |
| Event | Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 - Houston, TX, United States Duration: 2009 May 3 → 2009 May 7 |
Other
| Other | Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 |
|---|---|
| Country | United States |
| City | Houston, TX |
| Period | 09/5/3 → 09/5/7 |
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Keywords
- Au/ZnO
- Carbohydrate antigen 15.3
- Tumor marker
- Ultrasensitive
ASJC Scopus subject areas
- Biotechnology
- Biomedical Engineering
- Biomaterials
Cite this
Ultrasensitive protein detection using an Au/ZnO SPR-based biosensor. / Chang, C. C.; Lin, S. H.; Chiu, N. F.; Lin, C. W.
Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009. Vol. 2 2009. p. 172-175.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Ultrasensitive protein detection using an Au/ZnO SPR-based biosensor
AU - Chang, C. C.
AU - Lin, S. H.
AU - Chiu, N. F.
AU - Lin, C. W.
PY - 2009/12/1
Y1 - 2009/12/1
N2 - In this study, we demonstrated that the Au/ZnO nanocomposite films could be effectively used to enhance the performance of SPR for the detection of tumor marker. CA15.3 tumor marker for breast cancer was chosen as a model analyte. We analyzed experimentally intensity response to the samples that had different concentrations (0.0125 U/ml to 160 U/ml) in pleural fluid to evaluate the detection capability of the Au/ZnO SPR-based biosensor. As a result, it was found that the linear range extended from 0.025 to 40 U/ml with correlation coefficient of R = 0.9531 and the limit of detection at S/N of 3:1 reached 0.025U/ml. Compared with the shift degree of SPR intensity by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers increased at least 2 fold more than that on the Au/Cr layers. In addition, we determined that the Au/ZnO layers had a detection limit 4 times lower than the Au/Cr layers that were widespread use of the sensing interfaces on SPR. In conclusion, the use of Au/ZnO films greatly enhanced the SPR signal after carrying out bimolecular interactions and presented high sensitivity and reproducibility.
AB - In this study, we demonstrated that the Au/ZnO nanocomposite films could be effectively used to enhance the performance of SPR for the detection of tumor marker. CA15.3 tumor marker for breast cancer was chosen as a model analyte. We analyzed experimentally intensity response to the samples that had different concentrations (0.0125 U/ml to 160 U/ml) in pleural fluid to evaluate the detection capability of the Au/ZnO SPR-based biosensor. As a result, it was found that the linear range extended from 0.025 to 40 U/ml with correlation coefficient of R = 0.9531 and the limit of detection at S/N of 3:1 reached 0.025U/ml. Compared with the shift degree of SPR intensity by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers increased at least 2 fold more than that on the Au/Cr layers. In addition, we determined that the Au/ZnO layers had a detection limit 4 times lower than the Au/Cr layers that were widespread use of the sensing interfaces on SPR. In conclusion, the use of Au/ZnO films greatly enhanced the SPR signal after carrying out bimolecular interactions and presented high sensitivity and reproducibility.
KW - Au/ZnO
KW - Carbohydrate antigen 15.3
KW - Tumor marker
KW - Ultrasensitive
UR - http://www.scopus.com/inward/record.url?scp=77958030149&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77958030149&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:77958030149
SN - 9781439817834
SN - 9781439817834
VL - 2
SP - 172
EP - 175
BT - Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
ER -