Ultrasensitive protein detection using an Au/ZnO SPR-based biosensor

C. C. Chang, S. H. Lin, N. F. Chiu, C. W. Lin

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    In this study, we demonstrated that the Au/ZnO nanocomposite films could be effectively used to enhance the performance of SPR for the detection of tumor marker. CA15.3 tumor marker for breast cancer was chosen as a model analyte. We analyzed experimentally intensity response to the samples that had different concentrations (0.0125 U/ml to 160 U/ml) in pleural fluid to evaluate the detection capability of the Au/ZnO SPR-based biosensor. As a result, it was found that the linear range extended from 0.025 to 40 U/ml with correlation coefficient of R = 0.9531 and the limit of detection at S/N of 3:1 reached 0.025U/ml. Compared with the shift degree of SPR intensity by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers increased at least 2 fold more than that on the Au/Cr layers. In addition, we determined that the Au/ZnO layers had a detection limit 4 times lower than the Au/Cr layers that were widespread use of the sensing interfaces on SPR. In conclusion, the use of Au/ZnO films greatly enhanced the SPR signal after carrying out bimolecular interactions and presented high sensitivity and reproducibility.

    Original languageEnglish
    Title of host publicationTechnical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
    Pages172-175
    Number of pages4
    Volume2
    Publication statusPublished - 2009 Dec 1
    EventNanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 - Houston, TX, United States
    Duration: 2009 May 32009 May 7

    Other

    OtherNanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
    CountryUnited States
    CityHouston, TX
    Period09/5/309/5/7

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    Keywords

    • Au/ZnO
    • Carbohydrate antigen 15.3
    • Tumor marker
    • Ultrasensitive

    ASJC Scopus subject areas

    • Biotechnology
    • Biomedical Engineering
    • Biomaterials

    Cite this

    Chang, C. C., Lin, S. H., Chiu, N. F., & Lin, C. W. (2009). Ultrasensitive protein detection using an Au/ZnO SPR-based biosensor. In Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 (Vol. 2, pp. 172-175)