Ultrasensitive protein detection using an Au/ZnO SPR-based biosensor

C. C. Chang; S. H. Lin; N. F. Chiu; C. W. Lin

Ultrasensitive protein detection using an Au/ZnO SPR-based biosensor

C. C. Chang, S. H. Lin, N. F. Chiu, C. W. Lin

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this study, we demonstrated that the Au/ZnO nanocomposite films could be effectively used to enhance the performance of SPR for the detection of tumor marker. CA15.3 tumor marker for breast cancer was chosen as a model analyte. We analyzed experimentally intensity response to the samples that had different concentrations (0.0125 U/ml to 160 U/ml) in pleural fluid to evaluate the detection capability of the Au/ZnO SPR-based biosensor. As a result, it was found that the linear range extended from 0.025 to 40 U/ml with correlation coefficient of R = 0.9531 and the limit of detection at S/N of 3:1 reached 0.025U/ml. Compared with the shift degree of SPR intensity by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers increased at least 2 fold more than that on the Au/Cr layers. In addition, we determined that the Au/ZnO layers had a detection limit 4 times lower than the Au/Cr layers that were widespread use of the sensing interfaces on SPR. In conclusion, the use of Au/ZnO films greatly enhanced the SPR signal after carrying out bimolecular interactions and presented high sensitivity and reproducibility.

Original language	English
Title of host publication	Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
Pages	172-175
Number of pages	4
Volume	2
Publication status	Published - 2009 Dec 1
Event	Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 - Houston, TX, United States Duration: 2009 May 3 → 2009 May 7

Other

Other	Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
Country	United States
City	Houston, TX
Period	09/5/3 → 09/5/7

Keywords

Au/ZnO
Carbohydrate antigen 15.3
Tumor marker
Ultrasensitive

ASJC Scopus subject areas

Biotechnology
Biomedical Engineering
Biomaterials

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Chang, C. C., Lin, S. H., Chiu, N. F., & Lin, C. W. (2009). Ultrasensitive protein detection using an Au/ZnO SPR-based biosensor. In Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 (Vol. 2, pp. 172-175)

Chang, CC, Lin, SH, Chiu, NF & Lin, CW 2009, Ultrasensitive protein detection using an Au/ZnO SPR-based biosensor. in Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009. vol. 2, pp. 172-175, Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009, Houston, TX, United States, 09/5/3.

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abstract = "In this study, we demonstrated that the Au/ZnO nanocomposite films could be effectively used to enhance the performance of SPR for the detection of tumor marker. CA15.3 tumor marker for breast cancer was chosen as a model analyte. We analyzed experimentally intensity response to the samples that had different concentrations (0.0125 U/ml to 160 U/ml) in pleural fluid to evaluate the detection capability of the Au/ZnO SPR-based biosensor. As a result, it was found that the linear range extended from 0.025 to 40 U/ml with correlation coefficient of R = 0.9531 and the limit of detection at S/N of 3:1 reached 0.025U/ml. Compared with the shift degree of SPR intensity by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers increased at least 2 fold more than that on the Au/Cr layers. In addition, we determined that the Au/ZnO layers had a detection limit 4 times lower than the Au/Cr layers that were widespread use of the sensing interfaces on SPR. In conclusion, the use of Au/ZnO films greatly enhanced the SPR signal after carrying out bimolecular interactions and presented high sensitivity and reproducibility.",

keywords = "Au/ZnO, Carbohydrate antigen 15.3, Tumor marker, Ultrasensitive",

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year = "2009",

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AU - Lin, S. H.

AU - Chiu, N. F.

AU - Lin, C. W.

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AB - In this study, we demonstrated that the Au/ZnO nanocomposite films could be effectively used to enhance the performance of SPR for the detection of tumor marker. CA15.3 tumor marker for breast cancer was chosen as a model analyte. We analyzed experimentally intensity response to the samples that had different concentrations (0.0125 U/ml to 160 U/ml) in pleural fluid to evaluate the detection capability of the Au/ZnO SPR-based biosensor. As a result, it was found that the linear range extended from 0.025 to 40 U/ml with correlation coefficient of R = 0.9531 and the limit of detection at S/N of 3:1 reached 0.025U/ml. Compared with the shift degree of SPR intensity by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers increased at least 2 fold more than that on the Au/Cr layers. In addition, we determined that the Au/ZnO layers had a detection limit 4 times lower than the Au/Cr layers that were widespread use of the sensing interfaces on SPR. In conclusion, the use of Au/ZnO films greatly enhanced the SPR signal after carrying out bimolecular interactions and presented high sensitivity and reproducibility.

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