Ultrasensitive detection of nanoparticle-based bio bar-code DNA sensor by using nano-gap electrodes

Tien Li Chang*, Chien Ying Tsai, Ramesh Uppala, Chun Chi Chen, Chun Hung Lin, Ping Hei Chen

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

An ultra sensitive approach to detect the sub femtomolar DNA by using electrical detection method on a DNA biochip has been developed. The system provides high selectivity with sensitivity which is comparable to nano-gap-electrode detection approaches without the need of PCR amplification. Because bio-bar-code DNA approach is a pseudohomogeneous system with both magnetic nanoparticles and gold nanoparticles in solution, low concentration of the probes can be used very efficiently to bind target DNA, thereby reducing time required for high sensitivity detection experiments. Indeed, an advantage of the bio-bar-code DNA approach over conventional microarray sandwich assembly assays is that the entire self-assembly assay can be carried out in sub femtomolar concentration, regardless of target concentration. The system has an excellent dynamic range and is ideally set up for multiplexing.

Original languageEnglish
Title of host publicationDigest of Papers - Microprocesses and Nanotechnology 2005
Subtitle of host publication2005 International Microprocesses and Nanotechnology Conference
Pages210-211
Number of pages2
Publication statusPublished - 2005
Externally publishedYes
Event2005 International Microprocesses and Nanotechnology Conference - Tokyo, Japan
Duration: 2005 Oct 252005 Oct 28

Publication series

NameDigest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference
Volume2005

Other

Other2005 International Microprocesses and Nanotechnology Conference
Country/TerritoryJapan
CityTokyo
Period2005/10/252005/10/28

Keywords

  • Bio-bar-code DNA detection
  • DNA biochip
  • Electrical detection
  • Gold nanoparticles
  • Magnetic nanoparticles
  • Self-assembly

ASJC Scopus subject areas

  • General Engineering

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