Ultrasensitive Detection of Alzheimer's Amyloids on a Plasmonic-Gold Platform

Hsiao Fen Han, Hung Chi Yen, Hsiu Chuan Wu, Hsin Yuan Tan, Wei Xu, Hang Shiang Jiang, Ping Jui Tsai, Kun Qian, Yi Chun Wu*, Chia Chun Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


More than 55 million people live with dementia worldwide in 2021, and there are nearly 10 million new cases every year. Alzheimer's disease (AD) is the most common cause of dementia. Despite urgent need, early detection of AD and long-term monitoring of AD progression have been challenging. This is due to the limited availability of brain imaging facilities and the highly invasive procedure with the cerebrospinal fluid assay to assess the level of AD biomarkers, such as beta-amyloid (Aβ). Reliable measurements of AD biomarkers in blood samples are still difficult because of their very low abundance. Here, we develop a rapid, specific, and ultrasensitive immunoassay using plasmonic-gold nanoisland (pGOLD) chips with near-infrared fluorescence-enhanced detection for Aβ1-40 and Aβ1-42. We show step-by-step processes and results during the platform establishment, including antibody specificity and sensitivity tests, antibody pair examination, condition optimization, and procedure refinement. Finally, we demonstrate the platform performance with detection sensitivity at the subpicogram per milliliter level. This platform, therefore, has a great application potential for early detection of AD using blood samples.

Original languageEnglish
Pages (from-to)57036-57042
Number of pages7
JournalACS Applied Materials and Interfaces
Issue number48
Publication statusPublished - 2021 Dec 8


  • Alzheimer's disease
  • beta-amyloid
  • blood biomarkers
  • plasmonic-gold chip
  • surface plasmon-enhanced fluorescence

ASJC Scopus subject areas

  • General Materials Science


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