TY - JOUR
T1 - Ultra-low-temperature non-aqueous capillary electrophoretic separation-77 K fluorescence spectroscopic detection for the on-line identification of photo-converted analytes of trans-resveratrol
AU - Chen, Yi Hsiu
AU - Chung, Yu Lin
AU - Lin, Cheng Huang
N1 - Funding Information:
The authors wish to thank the National Science Council of Taiwan for financial support of this research under contract No. NSC-89-2113-M-003-016.
PY - 2002/1/18
Y1 - 2002/1/18
N2 - We demonstrate here, for the first time, that non-aqueous capillary electrophoresis (NACE) can be interfaced with any ultra-low-temperature (ULT) separation method and 77 K fluorescence spectroscopy (FS). This novel ULT-NACE-FS system consists of a modular CE system, a dry ice bath, and instrumentation for 77 K fluorescence detection. The ULT-NACE method serves to separate structurally similar molecules by a combination of a low electrophoresis current and a high voltage at ∼-70°C. When the ULT-NACE-separated analytes move into the quartz Dewar flask and traverse into the capillary detection window, liquid nitrogen was added, thus freezing the separating analyte zones, allowing the collection of 77 K fluorescence spectra for on-line spectral fingerprint identification. The first application of the ULT-NACE-FS system is described for the analysis of photo-converted analytes of trans-resveratrol; prospects and future applications of ULT-NACE-FS are also briefly addressed.
AB - We demonstrate here, for the first time, that non-aqueous capillary electrophoresis (NACE) can be interfaced with any ultra-low-temperature (ULT) separation method and 77 K fluorescence spectroscopy (FS). This novel ULT-NACE-FS system consists of a modular CE system, a dry ice bath, and instrumentation for 77 K fluorescence detection. The ULT-NACE method serves to separate structurally similar molecules by a combination of a low electrophoresis current and a high voltage at ∼-70°C. When the ULT-NACE-separated analytes move into the quartz Dewar flask and traverse into the capillary detection window, liquid nitrogen was added, thus freezing the separating analyte zones, allowing the collection of 77 K fluorescence spectra for on-line spectral fingerprint identification. The first application of the ULT-NACE-FS system is described for the analysis of photo-converted analytes of trans-resveratrol; prospects and future applications of ULT-NACE-FS are also briefly addressed.
KW - Capillary electrophoresis, ultra-low-temperature
KW - Fluorescence detection
KW - Non-aqueous capillary electrophoresis
KW - Phenolic compounds
KW - Resveratrol
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U2 - 10.1016/S0021-9673(01)01465-0
DO - 10.1016/S0021-9673(01)01465-0
M3 - Article
C2 - 11833648
AN - SCOPUS:0037126890
SN - 0021-9673
VL - 943
SP - 287
EP - 294
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -