Ultra-low-temperature non-aqueous capillary electrophoretic separation-77 K fluorescence spectroscopic detection for the on-line identification of photo-converted analytes of trans-resveratrol

Yi Hsiu Chen, Yu Lin Chung, Cheng-Huang Lin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We demonstrate here, for the first time, that non-aqueous capillary electrophoresis (NACE) can be interfaced with any ultra-low-temperature (ULT) separation method and 77 K fluorescence spectroscopy (FS). This novel ULT-NACE-FS system consists of a modular CE system, a dry ice bath, and instrumentation for 77 K fluorescence detection. The ULT-NACE method serves to separate structurally similar molecules by a combination of a low electrophoresis current and a high voltage at ∼-70°C. When the ULT-NACE-separated analytes move into the quartz Dewar flask and traverse into the capillary detection window, liquid nitrogen was added, thus freezing the separating analyte zones, allowing the collection of 77 K fluorescence spectra for on-line spectral fingerprint identification. The first application of the ULT-NACE-FS system is described for the analysis of photo-converted analytes of trans-resveratrol; prospects and future applications of ULT-NACE-FS are also briefly addressed.

Original languageEnglish
Pages (from-to)287-294
Number of pages8
JournalJournal of Chromatography A
Volume943
Issue number2
DOIs
Publication statusPublished - 2002 Jan 18

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Fluorescence
Fluorescence Spectrometry
Fluorescence spectroscopy
Temperature
Dry Ice
Dewars
Quartz
Dermatoglyphics
Liquid nitrogen
Electrophoresis
Baths
Freezing
resveratrol
Nitrogen
Molecules
Electric potential

Keywords

  • Capillary electrophoresis, ultra-low-temperature
  • Fluorescence detection
  • Non-aqueous capillary electrophoresis
  • Phenolic compounds
  • Resveratrol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

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title = "Ultra-low-temperature non-aqueous capillary electrophoretic separation-77 K fluorescence spectroscopic detection for the on-line identification of photo-converted analytes of trans-resveratrol",
abstract = "We demonstrate here, for the first time, that non-aqueous capillary electrophoresis (NACE) can be interfaced with any ultra-low-temperature (ULT) separation method and 77 K fluorescence spectroscopy (FS). This novel ULT-NACE-FS system consists of a modular CE system, a dry ice bath, and instrumentation for 77 K fluorescence detection. The ULT-NACE method serves to separate structurally similar molecules by a combination of a low electrophoresis current and a high voltage at ∼-70°C. When the ULT-NACE-separated analytes move into the quartz Dewar flask and traverse into the capillary detection window, liquid nitrogen was added, thus freezing the separating analyte zones, allowing the collection of 77 K fluorescence spectra for on-line spectral fingerprint identification. The first application of the ULT-NACE-FS system is described for the analysis of photo-converted analytes of trans-resveratrol; prospects and future applications of ULT-NACE-FS are also briefly addressed.",
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author = "Chen, {Yi Hsiu} and Chung, {Yu Lin} and Cheng-Huang Lin",
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AU - Chen, Yi Hsiu

AU - Chung, Yu Lin

AU - Lin, Cheng-Huang

PY - 2002/1/18

Y1 - 2002/1/18

N2 - We demonstrate here, for the first time, that non-aqueous capillary electrophoresis (NACE) can be interfaced with any ultra-low-temperature (ULT) separation method and 77 K fluorescence spectroscopy (FS). This novel ULT-NACE-FS system consists of a modular CE system, a dry ice bath, and instrumentation for 77 K fluorescence detection. The ULT-NACE method serves to separate structurally similar molecules by a combination of a low electrophoresis current and a high voltage at ∼-70°C. When the ULT-NACE-separated analytes move into the quartz Dewar flask and traverse into the capillary detection window, liquid nitrogen was added, thus freezing the separating analyte zones, allowing the collection of 77 K fluorescence spectra for on-line spectral fingerprint identification. The first application of the ULT-NACE-FS system is described for the analysis of photo-converted analytes of trans-resveratrol; prospects and future applications of ULT-NACE-FS are also briefly addressed.

AB - We demonstrate here, for the first time, that non-aqueous capillary electrophoresis (NACE) can be interfaced with any ultra-low-temperature (ULT) separation method and 77 K fluorescence spectroscopy (FS). This novel ULT-NACE-FS system consists of a modular CE system, a dry ice bath, and instrumentation for 77 K fluorescence detection. The ULT-NACE method serves to separate structurally similar molecules by a combination of a low electrophoresis current and a high voltage at ∼-70°C. When the ULT-NACE-separated analytes move into the quartz Dewar flask and traverse into the capillary detection window, liquid nitrogen was added, thus freezing the separating analyte zones, allowing the collection of 77 K fluorescence spectra for on-line spectral fingerprint identification. The first application of the ULT-NACE-FS system is described for the analysis of photo-converted analytes of trans-resveratrol; prospects and future applications of ULT-NACE-FS are also briefly addressed.

KW - Capillary electrophoresis, ultra-low-temperature

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KW - Non-aqueous capillary electrophoresis

KW - Phenolic compounds

KW - Resveratrol

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