Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays

S. Y. Yang; J. J. Chieh; W. C. Wang; C. Y. Yu; C. B. Lan; J. H. Chen; H. E. Horng; Chin Yih Hong; H. C. Yang; Wilber Huang

doi:10.1016/j.jviromet.2008.07.025

Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays

S. Y. Yang, J. J. Chieh, W. C. Wang, C. Y. Yu, C. B. Lan, J. H. Chen, H. E. Horng^*, Chin Yih Hong, H. C. Yang, Wilber Huang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

A platform for assaying avian influenza H5N1 viruses that involves measuring the ac immunomagnetic reduction of a magnetic reagent mixed with a detected sample is developed. The magnetic reagent contained magnetic nanoparticles coated with antibodies. To achieve an ultra-high sensitivity assay, a system utilizing a high-transition-temperature superconducting quantum interference device was used to sense the immunomagnetic reduction of the reagents. The results confirmed the ultra-high sensitivity of the immunomagnetic reduction assay on H5N1.

Original language	English
Pages (from-to)	250-252
Number of pages	3
Journal	Journal of Virological Methods
Volume	153
Issue number	2
DOIs	https://doi.org/10.1016/j.jviromet.2008.07.025
Publication status	Published - 2008 Nov

Keywords

H5N1
Immunomagnetic reduction
Magnetic nanoparticles
SQUID

ASJC Scopus subject areas

Virology

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10.1016/j.jviromet.2008.07.025

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title = "Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays",

abstract = "A platform for assaying avian influenza H5N1 viruses that involves measuring the ac immunomagnetic reduction of a magnetic reagent mixed with a detected sample is developed. The magnetic reagent contained magnetic nanoparticles coated with antibodies. To achieve an ultra-high sensitivity assay, a system utilizing a high-transition-temperature superconducting quantum interference device was used to sense the immunomagnetic reduction of the reagents. The results confirmed the ultra-high sensitivity of the immunomagnetic reduction assay on H5N1.",

keywords = "H5N1, Immunomagnetic reduction, Magnetic nanoparticles, SQUID",

author = "Yang, {S. Y.} and Chieh, {J. J.} and Wang, {W. C.} and Yu, {C. Y.} and Lan, {C. B.} and Chen, {J. H.} and Horng, {H. E.} and Hong, {Chin Yih} and Yang, {H. C.} and Wilber Huang",

note = "Funding Information: This work was supported by the National Science Council of Taiwan under Grant Nos. 95-2120-M-003-001, 95-2112-M-003-017-MY2, and 95-2752-M-002-016-PAE.",

year = "2008",

month = nov,

doi = "10.1016/j.jviromet.2008.07.025",

language = "English",

volume = "153",

pages = "250--252",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier",

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AU - Yang, S. Y.

AU - Chieh, J. J.

AU - Wang, W. C.

AU - Yu, C. Y.

AU - Lan, C. B.

AU - Chen, J. H.

AU - Horng, H. E.

AU - Hong, Chin Yih

AU - Yang, H. C.

AU - Huang, Wilber

N1 - Funding Information: This work was supported by the National Science Council of Taiwan under Grant Nos. 95-2120-M-003-001, 95-2112-M-003-017-MY2, and 95-2752-M-002-016-PAE.

PY - 2008/11

Y1 - 2008/11

N2 - A platform for assaying avian influenza H5N1 viruses that involves measuring the ac immunomagnetic reduction of a magnetic reagent mixed with a detected sample is developed. The magnetic reagent contained magnetic nanoparticles coated with antibodies. To achieve an ultra-high sensitivity assay, a system utilizing a high-transition-temperature superconducting quantum interference device was used to sense the immunomagnetic reduction of the reagents. The results confirmed the ultra-high sensitivity of the immunomagnetic reduction assay on H5N1.

AB - A platform for assaying avian influenza H5N1 viruses that involves measuring the ac immunomagnetic reduction of a magnetic reagent mixed with a detected sample is developed. The magnetic reagent contained magnetic nanoparticles coated with antibodies. To achieve an ultra-high sensitivity assay, a system utilizing a high-transition-temperature superconducting quantum interference device was used to sense the immunomagnetic reduction of the reagents. The results confirmed the ultra-high sensitivity of the immunomagnetic reduction assay on H5N1.

KW - H5N1

KW - Immunomagnetic reduction

KW - Magnetic nanoparticles

KW - SQUID

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SN - 0166-0934

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JO - Journal of Virological Methods

JF - Journal of Virological Methods

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ER -

Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays

Abstract

Keywords

ASJC Scopus subject areas

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