Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays

S. Y. Yang, J. J. Chieh, W. C. Wang, C. Y. Yu, C. B. Lan, J. H. Chen, H. E. Horng*, Chin Yih Hong, H. C. Yang, Wilber Huang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

A platform for assaying avian influenza H5N1 viruses that involves measuring the ac immunomagnetic reduction of a magnetic reagent mixed with a detected sample is developed. The magnetic reagent contained magnetic nanoparticles coated with antibodies. To achieve an ultra-high sensitivity assay, a system utilizing a high-transition-temperature superconducting quantum interference device was used to sense the immunomagnetic reduction of the reagents. The results confirmed the ultra-high sensitivity of the immunomagnetic reduction assay on H5N1.

Original languageEnglish
Pages (from-to)250-252
Number of pages3
JournalJournal of Virological Methods
Volume153
Issue number2
DOIs
Publication statusPublished - 2008 Nov

Keywords

  • H5N1
  • Immunomagnetic reduction
  • Magnetic nanoparticles
  • SQUID

ASJC Scopus subject areas

  • Virology

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