Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays

S. Y. Yang, J. J. Chieh, W. C. Wang, C. Y. Yu, C. B. Lan, J. H. Chen, H. E. Horng, Chin Yih Hong, H. C. Yang, Wilber Huang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A platform for assaying avian influenza H5N1 viruses that involves measuring the ac immunomagnetic reduction of a magnetic reagent mixed with a detected sample is developed. The magnetic reagent contained magnetic nanoparticles coated with antibodies. To achieve an ultra-high sensitivity assay, a system utilizing a high-transition-temperature superconducting quantum interference device was used to sense the immunomagnetic reduction of the reagents. The results confirmed the ultra-high sensitivity of the immunomagnetic reduction assay on H5N1.

Original languageEnglish
Pages (from-to)250-252
Number of pages3
JournalJournal of Virological Methods
Volume153
Issue number2
DOIs
Publication statusPublished - 2008 Nov 1

Keywords

  • H5N1
  • Immunomagnetic reduction
  • Magnetic nanoparticles
  • SQUID

ASJC Scopus subject areas

  • Virology

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