Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays

Shieh-Yueh Yang, Jen-Je Chieh, W. C. Wang, C. Y. Yu, C. B. Lan, J. H. Chen, Herng-Er Horng, Chin Yih Hong, Hong-Chang Yang, Wilber Huang

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    A platform for assaying avian influenza H5N1 viruses that involves measuring the ac immunomagnetic reduction of a magnetic reagent mixed with a detected sample is developed. The magnetic reagent contained magnetic nanoparticles coated with antibodies. To achieve an ultra-high sensitivity assay, a system utilizing a high-transition-temperature superconducting quantum interference device was used to sense the immunomagnetic reduction of the reagents. The results confirmed the ultra-high sensitivity of the immunomagnetic reduction assay on H5N1.

    Original languageEnglish
    Pages (from-to)250-252
    Number of pages3
    JournalJournal of Virological Methods
    Volume153
    Issue number2
    DOIs
    Publication statusPublished - 2008 Nov 1

    Fingerprint

    H5N1 Subtype Influenza A Virus
    Influenza in Birds
    Transition Temperature
    Orthomyxoviridae
    Nanoparticles
    Equipment and Supplies
    Antibodies

    Keywords

    • H5N1
    • Immunomagnetic reduction
    • Magnetic nanoparticles
    • SQUID

    ASJC Scopus subject areas

    • Virology

    Cite this

    Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays. / Yang, Shieh-Yueh; Chieh, Jen-Je; Wang, W. C.; Yu, C. Y.; Lan, C. B.; Chen, J. H.; Horng, Herng-Er; Hong, Chin Yih; Yang, Hong-Chang; Huang, Wilber.

    In: Journal of Virological Methods, Vol. 153, No. 2, 01.11.2008, p. 250-252.

    Research output: Contribution to journalArticle

    Yang, Shieh-Yueh ; Chieh, Jen-Je ; Wang, W. C. ; Yu, C. Y. ; Lan, C. B. ; Chen, J. H. ; Horng, Herng-Er ; Hong, Chin Yih ; Yang, Hong-Chang ; Huang, Wilber. / Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays. In: Journal of Virological Methods. 2008 ; Vol. 153, No. 2. pp. 250-252.
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    AU - Yu, C. Y.

    AU - Lan, C. B.

    AU - Chen, J. H.

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