Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays

S. Y. Yang, J. J. Chieh, W. C. Wang, C. Y. Yu, C. B. Lan, J. H. Chen, H. E. Horng, Chin Yih Hong, H. C. Yang, Wilber Huang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A platform for assaying avian influenza H5N1 viruses that involves measuring the ac immunomagnetic reduction of a magnetic reagent mixed with a detected sample is developed. The magnetic reagent contained magnetic nanoparticles coated with antibodies. To achieve an ultra-high sensitivity assay, a system utilizing a high-transition-temperature superconducting quantum interference device was used to sense the immunomagnetic reduction of the reagents. The results confirmed the ultra-high sensitivity of the immunomagnetic reduction assay on H5N1.

Original languageEnglish
Pages (from-to)250-252
Number of pages3
JournalJournal of Virological Methods
Volume153
Issue number2
DOIs
Publication statusPublished - 2008 Nov 1

Fingerprint

H5N1 Subtype Influenza A Virus
Influenza in Birds
Transition Temperature
Orthomyxoviridae
Nanoparticles
Equipment and Supplies
Antibodies

Keywords

  • H5N1
  • Immunomagnetic reduction
  • Magnetic nanoparticles
  • SQUID

ASJC Scopus subject areas

  • Virology

Cite this

Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays. / Yang, S. Y.; Chieh, J. J.; Wang, W. C.; Yu, C. Y.; Lan, C. B.; Chen, J. H.; Horng, H. E.; Hong, Chin Yih; Yang, H. C.; Huang, Wilber.

In: Journal of Virological Methods, Vol. 153, No. 2, 01.11.2008, p. 250-252.

Research output: Contribution to journalArticle

Yang, S. Y. ; Chieh, J. J. ; Wang, W. C. ; Yu, C. Y. ; Lan, C. B. ; Chen, J. H. ; Horng, H. E. ; Hong, Chin Yih ; Yang, H. C. ; Huang, Wilber. / Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays. In: Journal of Virological Methods. 2008 ; Vol. 153, No. 2. pp. 250-252.
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