Ultra-high sensitivity of the non-immunological affinity of graphene oxide-peptide-based surface plasmon resonance biosensors to detect human chorionic gonadotropin

Nan Fu Chiu, Chia Tzu Kuo, Ting Li Lin, Chia Chen Chang, Chen Yu Chen

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Specific peptide aptamers can be used in place of expensive antibody proteins, and they are gaining increasing importance as sensing probes due to their potential in the development of non-immunological assays with high sensitivity, affinity and specificity for human chorionic gonadotropin (hCG) protein. We combined graphene oxide (GO) sheets with a specific peptide aptamer to create a novel, simple and label-free tool to detect abnormalities at an early stage of pregnancy, a GO-peptide-based surface plasmon resonance (SPR) biosensor. This is the first binding interface experiment to successfully demonstrate binding specificity in kinetic analysis biomechanics in peptide aptamers and GO sheets. In addition to the improved affinity offered by the high compatibility with the target hCG protein, the major advantage of GO-peptide-based SPR sensors was their reduced nonspecific adsorption and enhanced sensitivity. The calculation of total electric field intensity (ΔE) in the GO-based sensing interfaces was significantly enhanced by up to 1.2 times that of a conventional SPR chip. The GO-peptide-based chip (1 mM) had a high affinity (KA) of 6.37×1012 M−1, limit of detection of 0.065 nM and ultra-high sensitivity of 16 times that of a conventional SPR chip. The sensitivity of the slope ratio of the low concentration hCG protein assay in linear regression analysis was GO-peptide (1 mM): GO-peptide (0.1 mM): conventional chip (8-mercaptooctanoic acid)-peptide (0.1 mM)=8.6: 3.3: 1. In summary, the excellent binding affinity, low detection limit, high sensitivity, good stability and specificity suggest the potential of this GO-peptide-based SPR chip detection method in clinical application. The development of real-time whole blood analytic and diagnostic tools to detect abnormalities at an early stage of pregnancy is a promising technique for future clinical application.

    Original languageEnglish
    Pages (from-to)351-357
    Number of pages7
    JournalBiosensors and Bioelectronics
    Volume94
    DOIs
    Publication statusPublished - 2017 Aug 15

    Fingerprint

    Graphite
    Surface Plasmon Resonance
    Surface plasmon resonance
    Biosensing Techniques
    Chorionic Gonadotropin
    Biosensors
    Oxides
    Graphene
    Peptides
    Peptide Aptamers
    Proteins
    Limit of Detection
    Assays
    Gonadotropins
    Pregnancy
    Biomechanics
    Biomechanical Phenomena
    Linear regression
    Regression analysis
    Adsorption

    Keywords

    • Graphene oxide (GO)
    • Human chorionic gonadotropin (hCG)
    • Peptide aptamer
    • Surface plasmon resonance (SPR)

    ASJC Scopus subject areas

    • Biotechnology
    • Biophysics
    • Biomedical Engineering
    • Electrochemistry

    Cite this

    Ultra-high sensitivity of the non-immunological affinity of graphene oxide-peptide-based surface plasmon resonance biosensors to detect human chorionic gonadotropin. / Chiu, Nan Fu; Kuo, Chia Tzu; Lin, Ting Li; Chang, Chia Chen; Chen, Chen Yu.

    In: Biosensors and Bioelectronics, Vol. 94, 15.08.2017, p. 351-357.

    Research output: Contribution to journalArticle

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    abstract = "Specific peptide aptamers can be used in place of expensive antibody proteins, and they are gaining increasing importance as sensing probes due to their potential in the development of non-immunological assays with high sensitivity, affinity and specificity for human chorionic gonadotropin (hCG) protein. We combined graphene oxide (GO) sheets with a specific peptide aptamer to create a novel, simple and label-free tool to detect abnormalities at an early stage of pregnancy, a GO-peptide-based surface plasmon resonance (SPR) biosensor. This is the first binding interface experiment to successfully demonstrate binding specificity in kinetic analysis biomechanics in peptide aptamers and GO sheets. In addition to the improved affinity offered by the high compatibility with the target hCG protein, the major advantage of GO-peptide-based SPR sensors was their reduced nonspecific adsorption and enhanced sensitivity. The calculation of total electric field intensity (ΔE) in the GO-based sensing interfaces was significantly enhanced by up to 1.2 times that of a conventional SPR chip. The GO-peptide-based chip (1 mM) had a high affinity (KA) of 6.37×1012 M−1, limit of detection of 0.065 nM and ultra-high sensitivity of 16 times that of a conventional SPR chip. The sensitivity of the slope ratio of the low concentration hCG protein assay in linear regression analysis was GO-peptide (1 mM): GO-peptide (0.1 mM): conventional chip (8-mercaptooctanoic acid)-peptide (0.1 mM)=8.6: 3.3: 1. In summary, the excellent binding affinity, low detection limit, high sensitivity, good stability and specificity suggest the potential of this GO-peptide-based SPR chip detection method in clinical application. The development of real-time whole blood analytic and diagnostic tools to detect abnormalities at an early stage of pregnancy is a promising technique for future clinical application.",
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    AU - Chang, Chia Chen

    AU - Chen, Chen Yu

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