Tyrosinase/Chitosan/Reduced graphene oxide modified screen-printed carbon electrode for sensitive and interference-free detection of dopamine

Cheng You Liu, Yi Chieh Chou, Jui Hsuan Tsai, Tzu Ming Huang, Jian Zhang Chen, Yi Chun Yeh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Tyrosinase, chitosan, and reduced graphene oxide (rGO) are sequentially used to modify a screen-printed carbon electrode (SPCE) for the detection of dopamine (DA), without interference from uric acid (UA) or ascorbic acid (AA). The use of tyrosinase significantly improves the detection's specificity. Cyclic voltammetry (CV) measurements demonstrate the high sensitivity and selectivity of the proposed electrochemical sensors, with detection limits of 22 nM and broad linear ranges of 0.4-8 μM and 40-500 μM. The fabricated tyrosinase/chitosan/rGO/SPCE electrodes achieve satisfactory results when applied to human urine samples, thereby demonstrating their feasibility for analyzing DA in physiological samples.

Original languageEnglish
Article number622
JournalApplied Sciences (Switzerland)
Volume9
Issue number4
DOIs
Publication statusPublished - 2019 Feb 13

Fingerprint

dopamine
Graphite
Monophenol Monooxygenase
Chitosan
Oxides
Graphene
Dopamine
graphene
Carbon
interference
Electrodes
electrodes
oxides
carbon
uric acid
Electrochemical sensors
urine
ascorbic acid
Ascorbic acid
Uric Acid

Keywords

  • Chitosan
  • Dopamine
  • Electrochemical biosensor
  • Reduced graphene oxide
  • Screen printing
  • Tyrosinase

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation
  • Engineering(all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes

Cite this

Tyrosinase/Chitosan/Reduced graphene oxide modified screen-printed carbon electrode for sensitive and interference-free detection of dopamine. / Liu, Cheng You; Chou, Yi Chieh; Tsai, Jui Hsuan; Huang, Tzu Ming; Chen, Jian Zhang; Yeh, Yi Chun.

In: Applied Sciences (Switzerland), Vol. 9, No. 4, 622, 13.02.2019.

Research output: Contribution to journalArticle

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AU - Chou, Yi Chieh

AU - Tsai, Jui Hsuan

AU - Huang, Tzu Ming

AU - Chen, Jian Zhang

AU - Yeh, Yi Chun

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