Two genetic divergence centers revealed by chloroplastic DNA variation in populations of Cinnamomum kanehirae Hay

Dai Chang Kuo, Chia Chia Lin, Kuo Chieh Ho, Yu Pin Cheng, Shih-Ying Hwang, Tsan Piao Lin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cinnamomum kanehirae Hayata (Lauraceae), the most valuable subtropical and temperate broadleaf timber tree in Taiwan, is rapidly disappearing from the wild. Taking advantage of a scion garden established by the Taiwan Forestry Research Institute, we examined patterns of chloroplast DNA (cpDNA) variations in 19 populations including 94 individuals. By sequencing two cpDNA fragments using universal primers (the trnL-trnF and petG-trnP intergenic spacers), we found eight polymorphic sites, six haplotypes, and extremely low nucleotide diversity (π = 0. 00016) from 792 bp aligned sequences. The ancestral haplotype is widely distributed. Among the populations studied, three separated populations, at Yungfeng, Fuli, and Tahu have high nucleotide diversity. No phylogeographical structures of haplotypes were revealed because the tests of NST-GST for populations did not differ from zero in any situations; a 'star-like' genealogy is characteristic when all haplotypes rapidly coalesce and is a general outcome of population expansion. The neutrality test also suggested demographic expansion. The genetic divergence and diversity analyses suggested that two potential refugia existed during the last glaciation with a major one located in southeastern Taiwan and a minor one located in Tahu in north-central Taiwan in the Hsuehshan Range, west of the Central Mountain Range.

Original languageEnglish
Pages (from-to)803-812
Number of pages10
JournalConservation Genetics
Volume11
Issue number3
DOIs
Publication statusPublished - 2010 Jun 1

Fingerprint

Cinnamomum
hay
chloroplast
Taiwan
divergence
Haplotypes
haplotypes
DNA
genetic variation
genealogy
last glaciation
Chloroplast DNA
refugium
Population
garden
timber
forestry
chloroplast DNA
Lauraceae
Nucleotides

Keywords

  • Chloroplast DNA
  • Cinnamomum kanehirae
  • Divergence center
  • Lauraceae
  • Refugia

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Two genetic divergence centers revealed by chloroplastic DNA variation in populations of Cinnamomum kanehirae Hay. / Kuo, Dai Chang; Lin, Chia Chia; Ho, Kuo Chieh; Cheng, Yu Pin; Hwang, Shih-Ying; Lin, Tsan Piao.

In: Conservation Genetics, Vol. 11, No. 3, 01.06.2010, p. 803-812.

Research output: Contribution to journalArticle

Kuo, Dai Chang ; Lin, Chia Chia ; Ho, Kuo Chieh ; Cheng, Yu Pin ; Hwang, Shih-Ying ; Lin, Tsan Piao. / Two genetic divergence centers revealed by chloroplastic DNA variation in populations of Cinnamomum kanehirae Hay. In: Conservation Genetics. 2010 ; Vol. 11, No. 3. pp. 803-812.
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