Trypsin-immobilized metal-organic framework as a biocatalyst in proteomics analysis

Yung Han Shih; Sheng Han Lo; Ni Shin Yang; Brenda Singco; Yi Jie Cheng; Cheng You Wu; I. Hsin Chang; Hsi Ya Huang; Chia Her Lin

doi:10.1002/cplu.201200186

Trypsin-immobilized metal-organic framework as a biocatalyst in proteomics analysis

Yung Han Shih, Sheng Han Lo, Ni Shin Yang, Brenda Singco, Yi Jie Cheng, Cheng You Wu, I. Hsin Chang, Hsi Ya Huang^*, Chia Her Lin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

147 Citations (Scopus)

Abstract

Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)-activated metal-organic frameworks (MOFs). After separation by nano-LCMS2 (liquid chromatography-mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin-MOF was comparable to traditional in-solution digestion (see figure). Furthermore, the trypsin-MOF was reusable.

Original language	English
Pages (from-to)	982-986
Number of pages	5
Journal	ChemPlusChem
Volume	77
Issue number	11
DOIs	https://doi.org/10.1002/cplu.201200186
Publication status	Published - 2012 Nov
Externally published	Yes

Keywords

Enzyme immobilization
Mass spectrometry
Metal-organic framework
Nanobiocatalysis
Proteomics

ASJC Scopus subject areas

General Chemistry

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10.1002/cplu.201200186

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title = "Trypsin-immobilized metal-organic framework as a biocatalyst in proteomics analysis",

abstract = "Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)-activated metal-organic frameworks (MOFs). After separation by nano-LCMS2 (liquid chromatography-mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin-MOF was comparable to traditional in-solution digestion (see figure). Furthermore, the trypsin-MOF was reusable.",

keywords = "Enzyme immobilization, Mass spectrometry, Metal-organic framework, Nanobiocatalysis, Proteomics",

author = "Shih, {Yung Han} and Lo, {Sheng Han} and Yang, {Ni Shin} and Brenda Singco and Cheng, {Yi Jie} and Wu, {Cheng You} and Chang, {I. Hsin} and Huang, {Hsi Ya} and Lin, {Chia Her}",

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AU - Shih, Yung Han

AU - Lo, Sheng Han

AU - Yang, Ni Shin

AU - Singco, Brenda

AU - Cheng, Yi Jie

AU - Wu, Cheng You

AU - Chang, I. Hsin

AU - Huang, Hsi Ya

AU - Lin, Chia Her

PY - 2012/11

Y1 - 2012/11

N2 - Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)-activated metal-organic frameworks (MOFs). After separation by nano-LCMS2 (liquid chromatography-mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin-MOF was comparable to traditional in-solution digestion (see figure). Furthermore, the trypsin-MOF was reusable.

AB - Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)-activated metal-organic frameworks (MOFs). After separation by nano-LCMS2 (liquid chromatography-mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin-MOF was comparable to traditional in-solution digestion (see figure). Furthermore, the trypsin-MOF was reusable.

KW - Enzyme immobilization

KW - Mass spectrometry

KW - Metal-organic framework

KW - Nanobiocatalysis

KW - Proteomics

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ER -

Trypsin-immobilized metal-organic framework as a biocatalyst in proteomics analysis

Abstract

Keywords

ASJC Scopus subject areas

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