Trypsin-immobilized metal-organic framework as a biocatalyst in proteomics analysis

Yung Han Shih, Sheng Han Lo, Ni Shin Yang, Brenda Singco, Yi Jie Cheng, Cheng You Wu, I. Hsin Chang, Hsi Ya Huang*, Chia Her Lin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

114 Citations (Scopus)

Abstract

Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)-activated metal-organic frameworks (MOFs). After separation by nano-LCMS2 (liquid chromatography-mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin-MOF was comparable to traditional in-solution digestion (see figure). Furthermore, the trypsin-MOF was reusable.

Original languageEnglish
Pages (from-to)982-986
Number of pages5
JournalChemPlusChem
Volume77
Issue number11
DOIs
Publication statusPublished - 2012 Nov
Externally publishedYes

Keywords

  • Enzyme immobilization
  • Mass spectrometry
  • Metal-organic framework
  • Nanobiocatalysis
  • Proteomics

ASJC Scopus subject areas

  • Chemistry(all)

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