Triterpenoids and polysaccharide fractions of Ganoderma tsugae exert different effects on antiallergic activities

Miaw Ling Chen, Chia Chien Hsieh, Bor Luen Chiang, Bi Fong Lin

Research output: Contribution to journalArticle

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Abstract

This study was to investigate antiallergic effects of triterpenoids (Gt-TRE) and polysaccharide (Gt-PS) extracts from Ganoderma tsugae, using mast cell line RBL-2H3, T cell line EL4, primary T cells, and transfected RAW264.7 macrophage cells. The results showed that histamine secreted from activated RBL-2H3 mast cells was significantly suppressed by Gt-TRE but not Gt-PS. Interleukin-(IL-) 4 secreted from activated EL4 cells was significantly suppressed by Gt-TRE but not Gt-PS. Further primary CD4+ T cells cultures also confirmed that Gt-TRE (5 50 μg/mL) significantly suppressed Th2 cytokines IL-4 and IL-5 secretions but had no effect on Th1 cytokines IL-2 and interferon (IFN)-γ. Gt-PS did not affect IL-4 and IL-5 secretions until higher doses (400, 500 μg/mL) and significantly suppressed IFNγ secretions but enhanced IL-2 at these high doses. The reporter gene assay indicated that Gt-TRE inhibited but Gt-PS enhanced the transcriptional activity of NF-B in activated transfected RAW264.7 cells and transfected EL4 cells. IL-4 secreted by this transfected EL-4 cells was also significantly decreased by Gt-TRE but not by Gt-PS, suggesting that these two fractions may exert different effects on NF-B related cytokines expression. These data suggested that triterpenoids fraction of Ganoderma tsugae might be the main constituents to alleviate allergic asthma.

Original languageEnglish
Article number754836
JournalEvidence-based Complementary and Alternative Medicine
Volume2015
DOIs
Publication statusPublished - 2015 Jan 1

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Ganoderma
Anti-Allergic Agents
Polysaccharides
Interleukin-4
Interleukin-5
Cytokines
T-Lymphocytes
Mast Cells
Interferons
Interleukin-2
Cell Line
Reporter Genes
Histamine
Asthma
Cell Culture Techniques
Macrophages

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Triterpenoids and polysaccharide fractions of Ganoderma tsugae exert different effects on antiallergic activities. / Chen, Miaw Ling; Hsieh, Chia Chien; Chiang, Bor Luen; Lin, Bi Fong.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2015, 754836, 01.01.2015.

Research output: Contribution to journalArticle

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