Transcriptomic Changes in Hot Spring Frog Tadpoles (Buergeria otai) in Response to Heat Stress

Shohei Komaki, Masatoshi Matsunami, Jhan Wei Lin, Ko Huan Lee, Yen Po Lin, Yu Lee, Si Min Lin, Takeshi Igawa*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Buergeria frog tadpoles exhibit high thermal tolerance and are occasionally found in water pools that temporarily exceed 40°C. With the aim of understanding how they can cope with the severe heat stress, we performed RNA-seq of three heat-treated (38°C) and three control (25°C) tadpoles and compared their transcriptomic profiles. We identified 382 differentially expressed transcripts. A protein-protein interaction (PPI) network analysis of these transcripts further identified hub proteins involved in protein degradation, stress granule assembly, and global suppression of DNA transcription and mRNA translation. Along with the avoidance behavior against high water temperature, these endurance mechanisms potentially support tadpoles to survive in high temperatures for short periods of time. Similar mechanisms may exist in many other amphibian species whose habitats are prone to high temperatures.

Original languageEnglish
Article number706887
JournalFrontiers in Ecology and Evolution
Publication statusPublished - 2021 Oct 13


  • RNA-seq
  • amphibia
  • anura
  • differentially expressed genes
  • heat stress response
  • high thermal tolerance
  • protein-protein interaction network

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology


Dive into the research topics of 'Transcriptomic Changes in Hot Spring Frog Tadpoles (Buergeria otai) in Response to Heat Stress'. Together they form a unique fingerprint.

Cite this