Transcription factor-based biosensor for detection of phenylalanine and tyrosine in urine for diagnosis of phenylketonuria

Chieh Lin, Yung Cheng Jair, Yi Chieh Chou, Pai Shan Chen, Yi Chun Yeh

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5 Citations (Scopus)

Abstract

Clinicians require a simple quantitative method for the detection of both phenylalanine and tyrosine to facilitate the diagnosis of phenylketonuria, a common inherited disorder of amino acid metabolism. In this study, we developed a novel whole-cell biosensor for the quantification of phenylalanine and tyrosine through the expression of red and green fluorescent proteins. The proposed system responds specifically and sensitively to phenylalanine/tyrosine without interferences from other amino acids. Furthermore, the precision of the biosensor was evaluated using specimens of normal human urine by LC-MS.

Original languageEnglish
Pages (from-to)108-113
Number of pages6
JournalAnalytica Chimica Acta
Volume1041
DOIs
Publication statusPublished - 2018 Dec 24

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Keywords

  • coli
  • E
  • Phenylalanine
  • Phenylketonuria
  • Tyrosine
  • tyrR

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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