Transcription factor-based biosensor for detection of phenylalanine and tyrosine in urine for diagnosis of phenylketonuria

Chieh Lin, Yung Cheng Jair, Yi Chieh Chou, Pai Shan Chen, Yi Chun Yeh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Clinicians require a simple quantitative method for the detection of both phenylalanine and tyrosine to facilitate the diagnosis of phenylketonuria, a common inherited disorder of amino acid metabolism. In this study, we developed a novel whole-cell biosensor for the quantification of phenylalanine and tyrosine through the expression of red and green fluorescent proteins. The proposed system responds specifically and sensitively to phenylalanine/tyrosine without interferences from other amino acids. Furthermore, the precision of the biosensor was evaluated using specimens of normal human urine by LC-MS.

Original languageEnglish
Pages (from-to)108-113
Number of pages6
JournalAnalytica Chimica Acta
Volume1041
DOIs
Publication statusPublished - 2018 Dec 24

Fingerprint

Phenylketonurias
Biosensing Techniques
Phenylalanine
Biosensors
urine
Tyrosine
Transcription Factors
amino acid
Urine
Inborn Errors Amino Acid Metabolism
Amino Acids
metabolism
Green Fluorescent Proteins
Metabolism
protein
detection
method

Keywords

  • coli
  • E
  • Phenylalanine
  • Phenylketonuria
  • Tyrosine
  • tyrR

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Transcription factor-based biosensor for detection of phenylalanine and tyrosine in urine for diagnosis of phenylketonuria. / Lin, Chieh; Jair, Yung Cheng; Chou, Yi Chieh; Chen, Pai Shan; Yeh, Yi Chun.

In: Analytica Chimica Acta, Vol. 1041, 24.12.2018, p. 108-113.

Research output: Contribution to journalArticle

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AU - Yeh, Yi Chun

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