Titanium dioxide nanoparticles induce emphysema-like lung injury in mice

Huei Wen Chen, Sheng Fang Su, Chiang-Ting Chien, Wei Hsiang Lin, Sung Liang Yu, Cheng Chung Chou, Jeremy J.W. Chen, Pan Chyr Yang

Research output: Contribution to journalArticle

263 Citations (Scopus)

Abstract

Titanium dioxide nanoparticles (nano-TiO 2 ) have been widely used as a photocatalyst in air and water cleaning. However, these nanoparticles inhalation can induce pulmonary toxicity and its mechanism is not fully understood. In this study we investigated the pulmonary toxicity of nanoTiO 2 and its molecular pathogenesis. The adult male ICR mice were exposed to intratracheal single dose of 0.1 or 0.5 mg nanoTiO 2 (19-21 nm) and lung tissues were collected at 3rd day, 1st wk, and 2nd wk for morphometric, microarray gene expression, and pathway analyses. NanoTiO 2 can induce pulmonary emphysema, macrophages accumulation, extensive disruption of alveolar septa, type II pneumocyte hyperplasia, and epithelial cell apoptosis. NanoTiO 2 induced differential expression of hundreds of genes include activation of pathways involved in cell cycle, apoptosis, chemokines, and complement cascades. In particular, nanoTiO 2 up-regulates placenta growth factor (PlGF) and other chemokines (CXCL1, CXCL5, and CCL3) expressions that may cause pulmonary emphysema and alveolar epithelial cell apoptosis. Cultured human THP-1 cell-derived macrophages treated with nanoTiO 2 in vitro also resulted in upregulations of PlGF, CXCL1, CXCL5, and CCL3. These results indicated that nanoTiO 2 can induce severe pulmonary emphysema, which may be caused by activation of PlGF and related inflammatory pathways.

Original languageEnglish
JournalFASEB Journal
Volume20
Issue number13
DOIs
Publication statusPublished - 2006 Nov 1

Fingerprint

Pulmonary Emphysema
Emphysema
Lung Injury
Nanoparticles
Alveolar Epithelial Cells
Intercellular Signaling Peptides and Proteins
Apoptosis
Lung
Chemokine CXCL5
Toxicity
Chemokine CXCL1
Up-Regulation
Chemical activation
Chemokine CCL3
Inbred ICR Mouse
Macrophages
Alveolar Macrophages
Microarrays
Photocatalysts
Chemokines

Keywords

  • Chemokines
  • Microarray
  • Nanotechnology
  • Placenta growth factor
  • Pulmonary emphysema

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Chen, H. W., Su, S. F., Chien, C-T., Lin, W. H., Yu, S. L., Chou, C. C., ... Yang, P. C. (2006). Titanium dioxide nanoparticles induce emphysema-like lung injury in mice. FASEB Journal, 20(13). https://doi.org/10.1096/fj.06-6485fje

Titanium dioxide nanoparticles induce emphysema-like lung injury in mice. / Chen, Huei Wen; Su, Sheng Fang; Chien, Chiang-Ting; Lin, Wei Hsiang; Yu, Sung Liang; Chou, Cheng Chung; Chen, Jeremy J.W.; Yang, Pan Chyr.

In: FASEB Journal, Vol. 20, No. 13, 01.11.2006.

Research output: Contribution to journalArticle

Chen, HW, Su, SF, Chien, C-T, Lin, WH, Yu, SL, Chou, CC, Chen, JJW & Yang, PC 2006, 'Titanium dioxide nanoparticles induce emphysema-like lung injury in mice', FASEB Journal, vol. 20, no. 13. https://doi.org/10.1096/fj.06-6485fje
Chen, Huei Wen ; Su, Sheng Fang ; Chien, Chiang-Ting ; Lin, Wei Hsiang ; Yu, Sung Liang ; Chou, Cheng Chung ; Chen, Jeremy J.W. ; Yang, Pan Chyr. / Titanium dioxide nanoparticles induce emphysema-like lung injury in mice. In: FASEB Journal. 2006 ; Vol. 20, No. 13.
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