Time-dependent phase lag of biofunctionalized magnetic nanoparticles conjugated with biotargets studied with alternating current magnetic susceptometor for liquid phase immunoassays

S. H. Liao; H. C. Yang; H. E. Horng; J. J. Chieh; K. L. Chen; H. H. Chen; J. Y. Chen; C. I. Liu; C. W. Liu; L. M. Wang

doi:10.1063/1.4846536

Time-dependent phase lag of biofunctionalized magnetic nanoparticles conjugated with biotargets studied with alternating current magnetic susceptometor for liquid phase immunoassays

S. H. Liao^*, H. C. Yang, H. E. Horng, J. J. Chieh, K. L. Chen, H. H. Chen, J. Y. Chen, C. I. Liu, C. W. Liu, L. M. Wang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

In this work, the time-dependent phase lag θ of biofunctionalized magnetic nanoparticles (BMNs) conjugated with biotargets is studied with a home-made alternating current (ac) susceptometor for liquid phase immunoassays. The sensing unit of the ac susceptometor composed of excitation, pick-up, and compensation coils are balanced to 0.03 ppm. The BMNs are anti-goat C-reactive protein coated onto dextran-coated magnetic nanoparticles composed of Fe ₃O₄, labeled as Fe₃O₄-antiCRP. The bio-targets are human CRP. As the human CRP is conjugated with reagents Fe ₃O₄-antiCRP, the magnetic clusters of Fe₃O ₄-antiCRP-CRP are formulated. Due to the clustering effect, the Brownian relaxation of BMNs will be depressed, which in turn enhances the effective relaxation time. By monitoring the dynamic phase lag, we demonstrate a sensitive platform of assaying human CRP. The detection platform is robust, easy to use and can be applied for assaying a wide variety of biotargets including viruses, proteins, tumor markers, chemicals, etc.

Original language	English
Article number	243703
Journal	Applied Physics Letters
Volume	103
Issue number	24
DOIs	https://doi.org/10.1063/1.4846536
Publication status	Published - 2013 Dec 9

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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Liao, S. H., Yang, H. C., Horng, H. E., Chieh, J. J., Chen, K. L., Chen, H. H., Chen, J. Y., Liu, C. I., Liu, C. W., & Wang, L. M. (2013). Time-dependent phase lag of biofunctionalized magnetic nanoparticles conjugated with biotargets studied with alternating current magnetic susceptometor for liquid phase immunoassays. Applied Physics Letters, 103(24), Article 243703. https://doi.org/10.1063/1.4846536

Liao, SH, Yang, HC, Horng, HE , Chieh, JJ, Chen, KL, Chen, HH, Chen, JY, Liu, CI, Liu, CW & Wang, LM 2013, 'Time-dependent phase lag of biofunctionalized magnetic nanoparticles conjugated with biotargets studied with alternating current magnetic susceptometor for liquid phase immunoassays', Applied Physics Letters, vol. 103, no. 24, 243703. https://doi.org/10.1063/1.4846536

@article{7565469afb8a491d87ba0783cd79a517,

title = "Time-dependent phase lag of biofunctionalized magnetic nanoparticles conjugated with biotargets studied with alternating current magnetic susceptometor for liquid phase immunoassays",

abstract = "In this work, the time-dependent phase lag θ of biofunctionalized magnetic nanoparticles (BMNs) conjugated with biotargets is studied with a home-made alternating current (ac) susceptometor for liquid phase immunoassays. The sensing unit of the ac susceptometor composed of excitation, pick-up, and compensation coils are balanced to 0.03 ppm. The BMNs are anti-goat C-reactive protein coated onto dextran-coated magnetic nanoparticles composed of Fe 3O4, labeled as Fe3O4-antiCRP. The bio-targets are human CRP. As the human CRP is conjugated with reagents Fe 3O4-antiCRP, the magnetic clusters of Fe3O 4-antiCRP-CRP are formulated. Due to the clustering effect, the Brownian relaxation of BMNs will be depressed, which in turn enhances the effective relaxation time. By monitoring the dynamic phase lag, we demonstrate a sensitive platform of assaying human CRP. The detection platform is robust, easy to use and can be applied for assaying a wide variety of biotargets including viruses, proteins, tumor markers, chemicals, etc.",

author = "Liao, {S. H.} and Yang, {H. C.} and Horng, {H. E.} and Chieh, {J. J.} and Chen, {K. L.} and Chen, {H. H.} and Chen, {J. Y.} and Liu, {C. I.} and Liu, {C. W.} and Wang, {L. M.}",

note = "Funding Information: This work was supported by National Science Council of Taiwan under Grant Nos. 101-2811-M-168-001, 102-2120-M-168-001, 102-2112-M-168-001, 102-2112-M-003-017, 101-2112-M-003-009, 102-2923-M003-001, 102-2622-M002-001-CC2, 102-2112-M-003-008-MY2, 100-2112-M-003-011-MY2 and 99-2112-M-168-001-MY3, the Ministry of Health and Welfare under Grant No. DOH102-TD-PB-111-TM007 and DOH102-TD-N-111-002.",

year = "2013",

month = dec,

day = "9",

doi = "10.1063/1.4846536",

language = "English",

volume = "103",

journal = "Applied Physics Letters",

issn = "0003-6951",

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T1 - Time-dependent phase lag of biofunctionalized magnetic nanoparticles conjugated with biotargets studied with alternating current magnetic susceptometor for liquid phase immunoassays

AU - Liao, S. H.

AU - Yang, H. C.

AU - Horng, H. E.

AU - Chieh, J. J.

AU - Chen, K. L.

AU - Chen, H. H.

AU - Chen, J. Y.

AU - Liu, C. I.

AU - Liu, C. W.

AU - Wang, L. M.

N1 - Funding Information: This work was supported by National Science Council of Taiwan under Grant Nos. 101-2811-M-168-001, 102-2120-M-168-001, 102-2112-M-168-001, 102-2112-M-003-017, 101-2112-M-003-009, 102-2923-M003-001, 102-2622-M002-001-CC2, 102-2112-M-003-008-MY2, 100-2112-M-003-011-MY2 and 99-2112-M-168-001-MY3, the Ministry of Health and Welfare under Grant No. DOH102-TD-PB-111-TM007 and DOH102-TD-N-111-002.

PY - 2013/12/9

Y1 - 2013/12/9

N2 - In this work, the time-dependent phase lag θ of biofunctionalized magnetic nanoparticles (BMNs) conjugated with biotargets is studied with a home-made alternating current (ac) susceptometor for liquid phase immunoassays. The sensing unit of the ac susceptometor composed of excitation, pick-up, and compensation coils are balanced to 0.03 ppm. The BMNs are anti-goat C-reactive protein coated onto dextran-coated magnetic nanoparticles composed of Fe 3O4, labeled as Fe3O4-antiCRP. The bio-targets are human CRP. As the human CRP is conjugated with reagents Fe 3O4-antiCRP, the magnetic clusters of Fe3O 4-antiCRP-CRP are formulated. Due to the clustering effect, the Brownian relaxation of BMNs will be depressed, which in turn enhances the effective relaxation time. By monitoring the dynamic phase lag, we demonstrate a sensitive platform of assaying human CRP. The detection platform is robust, easy to use and can be applied for assaying a wide variety of biotargets including viruses, proteins, tumor markers, chemicals, etc.

AB - In this work, the time-dependent phase lag θ of biofunctionalized magnetic nanoparticles (BMNs) conjugated with biotargets is studied with a home-made alternating current (ac) susceptometor for liquid phase immunoassays. The sensing unit of the ac susceptometor composed of excitation, pick-up, and compensation coils are balanced to 0.03 ppm. The BMNs are anti-goat C-reactive protein coated onto dextran-coated magnetic nanoparticles composed of Fe 3O4, labeled as Fe3O4-antiCRP. The bio-targets are human CRP. As the human CRP is conjugated with reagents Fe 3O4-antiCRP, the magnetic clusters of Fe3O 4-antiCRP-CRP are formulated. Due to the clustering effect, the Brownian relaxation of BMNs will be depressed, which in turn enhances the effective relaxation time. By monitoring the dynamic phase lag, we demonstrate a sensitive platform of assaying human CRP. The detection platform is robust, easy to use and can be applied for assaying a wide variety of biotargets including viruses, proteins, tumor markers, chemicals, etc.

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IS - 24

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ER -

Time-dependent phase lag of biofunctionalized magnetic nanoparticles conjugated with biotargets studied with alternating current magnetic susceptometor for liquid phase immunoassays

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