Three dimensional electrode array for cell lysis via electroporation

Kuan Ying Lu, Andrew M. Wo, Ying Jie Lo, Ken Chao Chen, Cheng Ming Lin, Chii Rong Yang

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Microfabricated devices for cell lysis have demonstrated many advantages over conventional approaches. Among various design of microdevices that employ electroporation for cytolysis, most utilize Ag/AgCl wires or 2D planar electrodes. Although, simple in fabrication the electric field generated by 2D electrodes decays exponentially, resulting in rather non-uniform forcing on the cell membrane. This paper investigates the effect of electric field generated by 3D cylindrical electrodes to perform cell lysis via electroporation in a microfluidic platform, and compared with that by 2D design. Computational results of the electric field for both 2D and 3D electrode geometries showed that the 3D configuration demonstrated a significantly higher effective volume ratio-volume which electric field is sufficient for cell lysis to that of net throughflow volume. Hence, the efficacy of performing cell lysis is substantially greater for cells passing through 3D than 2D electrodes. Experimentally, simultaneous multi-pores were observed on leukocytes lysed with 3D electrodes, which is indicative of enhanced uniformity of the electric field generated by 3D design. Additionally, a single row of 3D electrode demonstrated a substantially higher lysing percentage (30%) than that of 2D (8%) under that same flow condition. This work should aid in the design of electrodes in performing cell lysis via electroporation.

Original languageEnglish
Pages (from-to)568-574
Number of pages7
JournalBiosensors and Bioelectronics
Volume22
Issue number4 SPEC. ISS.
DOIs
Publication statusPublished - 2006 Oct 15

Fingerprint

Electroporation
Electrodes
Electric fields
Microfluidics
Cell membranes
Leukocytes
Cell Membrane
Wire
Fabrication
Equipment and Supplies
Geometry

Keywords

  • Cell lysis
  • Electrode design
  • Electroporation
  • Microfluidics

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Three dimensional electrode array for cell lysis via electroporation. / Lu, Kuan Ying; Wo, Andrew M.; Lo, Ying Jie; Chen, Ken Chao; Lin, Cheng Ming; Yang, Chii Rong.

In: Biosensors and Bioelectronics, Vol. 22, No. 4 SPEC. ISS., 15.10.2006, p. 568-574.

Research output: Contribution to journalArticle

Lu, Kuan Ying ; Wo, Andrew M. ; Lo, Ying Jie ; Chen, Ken Chao ; Lin, Cheng Ming ; Yang, Chii Rong. / Three dimensional electrode array for cell lysis via electroporation. In: Biosensors and Bioelectronics. 2006 ; Vol. 22, No. 4 SPEC. ISS. pp. 568-574.
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