The use of a gas chromatography/milli-whistle technique for the on-line monitoring of ethanol production using microtube array membrane immobilized yeast cells

Ming Ju Wu, Guan Fu Ye, Ching Hao Wang, Hong Ting Victor Lin, Chien Chung Chen, Cheng-Huang Lin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Hollow, poly(L-lactic acid) microtube array membranes (MTAM) were used in preparing membranes that contained immobilized yeast cells. To evaluate the performance of the developed system for continuous and fed-batch fermentation, a gas chromatography/milli-whistle device was used to on-line monitor the production of ethanol. The milli-whistle was connected to the outlet of a GC capillary, and when the fermentation gases and the GC carrier gas passed through it, a sound with a fundamental frequency was produced. The online data obtained for frequency-change vs. retention time can be recorded after a fast Fourier transform. In typical bioethanol fermentation, the yeast cells cannot be recycled, whereas the artificial yeast-MTAMs can be. The hollow-MTAM containing immobilized yeast cells significantly enhanced to bioethanol productivity, and represent a novel, promising technology for bioethanol fermentation. Our data indicate that the gas chromatography/milli-whistle device, which is economical and stable, is a very useful detector for long-term monitoring.

Original languageEnglish
Pages (from-to)625-630
Number of pages6
JournalAnalytical Sciences
Volume33
Issue number5
DOIs
Publication statusPublished - 2017 Jan 1

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Gas chromatography
Yeast
Fermentation
Bioethanol
Ethanol
Cells
Membranes
Monitoring
Gases
Fast Fourier transforms
Productivity
Acoustic waves
Detectors

Keywords

  • Ethanol
  • Gas chromatography
  • Whistle
  • Yeast immobilization
  • Yeast-MTAM

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

The use of a gas chromatography/milli-whistle technique for the on-line monitoring of ethanol production using microtube array membrane immobilized yeast cells. / Wu, Ming Ju; Ye, Guan Fu; Wang, Ching Hao; Lin, Hong Ting Victor; Chen, Chien Chung; Lin, Cheng-Huang.

In: Analytical Sciences, Vol. 33, No. 5, 01.01.2017, p. 625-630.

Research output: Contribution to journalArticle

Wu, Ming Ju ; Ye, Guan Fu ; Wang, Ching Hao ; Lin, Hong Ting Victor ; Chen, Chien Chung ; Lin, Cheng-Huang. / The use of a gas chromatography/milli-whistle technique for the on-line monitoring of ethanol production using microtube array membrane immobilized yeast cells. In: Analytical Sciences. 2017 ; Vol. 33, No. 5. pp. 625-630.
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